ARP3/ARP3B Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

ARP3/ARP3B Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

11

Kat-Nr. : 13822-1-AP

Synonyme

Actin like protein 3, Actin related protein 3, ACTR3, ACTR3B, Arp11, ARP3, ARP3/ARP3B, Arp4

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Galerie der Validierungsdaten

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  WB analysis using 13822-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13822-1-AP (ARP3/ARP3B antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 13822-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 13822-1-AP (ARP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 13822-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 13822-1-AP (ARP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse spleen using 13822-1-AP

  IP Result of anti-ARP3 (IP:13822-1-AP, 4ug; Detection:13822-1-AP 1:1000) with mouse spleen tissue lysate 4000ug.

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  IHC staining of human kidney using 13822-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13822-1-AP (ARP3/ARP3B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, A549-Zellen, HeLa-Zellen, HL-60-Zellen, humanes Lebergewebe, Jurkat-Zellen, K-562-Zellen, Mausmilzgewebe, PC-3-Zellen
• Erfolgreiche IP: Mausmilzgewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IF: See 4 publications below
• IP: See 1 publications below

Produktinformation

13822-1-AP bindet in WB, IP, IHC, IF, ELISA ARP3/ARP3B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ARP3/ARP3B fusion protein Ag4922
• Vollständiger Name: ARP3 actin-related protein 3 homolog (yeast)
• Berechnetes Molekulargewicht: 418 aa 47 kDa
• Beobachtetes Molekulargewicht: 47 kDa
• GenBank-Zugangsnummer: BC044590
• Gene symbol: ACTR3
• Gene ID (NCBI): 10096
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ARP3 (also known as ACTR3) is a major constituent of the ARP2/3 complex that is necessary for nucleating actin polymerization at filament branches. ARP3 localizes to the lamellipodia of stationary and locomoting fibroblasts. Overexpression of ARP3 has been reported in several types of cancer cells. This antibody recognizes endogenous ARP3 and may cross-react with ATCR3B.

Publikationen

Species	Application	Title
human	WB	Nature; Actin cables and comet tails organize mitochondrial networks in mitosis.; Authors - Andrew S Moore
rat	WB,IF	ACS Nano; Oxidation of Reduced Graphene Oxide via Cellular Redox Signaling Modulates Actin-Mediated Neurotransmission.; Authors - Yiyuan Kang
mouse	IF	PLoS Genet; N-wasp is required for structural integrity of the blood-testis barrier.; Authors - Xiang Xiao
human	IP	EMBO Rep; WDR63 inhibits Arp2/3-dependent actin polymerization and mediates the function of p53 in suppressing metastasis.; Authors - Kailiang Zhao
	WB	Elife; Syncytin-mediated open-ended membrane tubular connections facilitate the intercellular transfer of cargos including Cas9 protein; Authors - Congyan Zhang
human	WB	Int J Biol Sci; Rictor promotes cell migration and actin polymerization through regulating ABLIM1 phosphorylation in Hepatocellular Carcinoma.; Authors - Xin Dong