ACTR3B Monoklonaler Antikörper

ACTR3B Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1G12C6

Kat-Nr. : 68037-1-PBS

Synonyme

1G12C6, Actin like protein 3B, Actin related protein 3B, Actin related protein ARP4, Actin-like protein 3B

---

• 

  WB analysis using 68037-1-Ig (same clone as 68037-1-PBS)

  Various cells were subjected to SDS PAGE followed by western blot with 68037-1-Ig (ACTR3B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68037-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68037-1-Ig (same clone as 68037-1-PBS)

  Brain tissues were subjected to SDS PAGE followed by western blot with 68037-1-Ig (ACTR3B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68037-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 68037-1-Ig (same clone as 68037-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68037-1-Ig (ACTR3B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68037-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 68037-1-Ig (same clone as 68037-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68037-1-Ig (ACTR3B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68037-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 68037-1-Ig (same clone as 68037-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ACTR3B antibody (68037-1-Ig, Clone: 1G12C6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68037-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

68037-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA ACTR3B und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ACTR3B fusion protein Ag10218
• Vollständiger Name: ARP3 actin-related protein 3 homolog B (yeast)
• Berechnetes Molekulargewicht: 418 aa, 48 kDa
• Beobachtetes Molekulargewicht: 48 kDa
• GenBank-Zugangsnummer: BC008682
• Gene symbol: ACTR3B
• Gene ID (NCBI): 57180
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.