ACVR1 Monoklonaler Antikörper

ACVR1 Monoklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

1F11B10

Kat-Nr. : 67417-1-Ig

Synonyme

activin A receptor, type I, Activin receptor like kinase 2, Activin receptor type 1, Activin receptor type I, ACTR I, ACTRI, ACVR1, ACVR1A, ACVRLK2, ALK 2, ALK2, FOP, SKR1, TSR I, TSRI

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  WB analysis using 67417-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67417-1-Ig (ACVR1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 67417-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 67417-1-Ig (ACVR1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig brain using 67417-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67417-1-Ig (ACVR1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67417-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67417-1-Ig (ACVR1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse heart using 67417-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 67417-1-Ig (ACVR1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 67417-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 67417-1-Ig (ACVR1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 67417-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67417-1-Ig (ACVR1 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 67417-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67417-1-Ig (ACVR1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 67417-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using ACVR1 antibody (67417-1-Ig, Clone: 1F11B10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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  IF Staining of mouse brain using 67417-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using ACVR1 antibody (67417-1-Ig, Clone: 1F11B10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Hausschwein-Hirngewebe, JAR-Zellen, Maushirngewebe, NCI-H1299-Zellen, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: Mausherzgewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:150-1:600
• Immunfluoreszenz (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

67417-1-Ig bindet in WB, IHC, IF-P, ELISA ACVR1 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ACVR1 fusion protein Ag13508
• Vollständiger Name: activin A receptor, type I
• Berechnetes Molekulargewicht: 509 aa, 57 kDa
• Beobachtetes Molekulargewicht: 57 kDa
• GenBank-Zugangsnummer: BC033867
• Gene symbol: ACVR1
• Gene ID (NCBI): 90
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ACVR1 (activin receptor type I), also known as ALK2 or ACTRI, is a receptor for activin. It forms a stable complex with type II receptor after ligand binding. These receptors are all transmembrane proteins, composed of a ligand-binding extracellular domain with cysteine-rich region, a transmembrane domain, and a cytoplasmic domain with predicted serine/threonine specificity. Type I receptors are essential for signaling, and type II receptors are required for binding ligands and for expression of type I receptors. ACVR1 is expressed in many tissues including skeletal muscle and chondrocytes. It functions as a receptor for bone morphogenetic protein (BMP) and induces Indian hedgehog in chondrocytes during skeletal development. Mutations in ACVR1 gene are associated with fibrodysplasia ossificans progressive (PMID: 16642017).

Publikationen

Species	Application	Title
human	WB	J Cell Mol Med; Circulating exosomes repair endothelial cell damage by delivering miR-193a-5p.; Authors - Chang Cao