ACVRL1 Rekombinanter Antikörper

ACVRL1 Rekombinant Antikörper für IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

241686B9

Kat-Nr. : 84421-1-PBS

Synonyme

241686B9, Activin receptor like kinase 1, Activin receptor-like kinase 1, ACVRLK1, ALK 1

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  Sandwich ELISA standard curve of MP01306-2

  Sandwich ELISA standard curve of MP01306-2, Human ACVRL1 Recombinant Matched Antibody Pair - PBS only. 84421-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg2132. 84421-2-PBS was HRP conjugated as the detection antibody. Range: 0.781-50 ng/mL.

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  Sample test of MP01306-2

  The mean ACVRL1 concentration was determined to be 34.48 ng/mL in HUVEC cell extract based on a 0.91 mg/mL extract load, 1108.74 ng/mL in HL-60 cell extract based on a 3.00 mg/mL extract load and 176.59 ng/mL in U-937 cell extract based on a 1.40 mg/mL extract load.

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  IF Staining of Jurkat using 84421-1-RR (same clone as 84421-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using ACVRL1 antibody (84421-1-RR, Clone: 241686B9 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84421-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84421-1-PBS

  Biolayer interferometry (BLl) kinetic assay of 84421-1-PBS against Human ACVRL1 was performed. The affinity constant is 3.24 nM.

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  Affinity and Kinetic Characterization of 84421-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84421-1-RR against Human ACVRL1 were performed. The affinity constant is 0.727 nM.

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Geprüfte Anwendungen

Produktinformation

84421-1-PBS bindet in IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test ACVRL1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ACVRL1 fusion protein Eg2132
• Vollständiger Name: activin A receptor type II-like 1
• Berechnetes Molekulargewicht: 56 kDa
• GenBank-Zugangsnummer: BC042637
• Gene symbol: ACVRL1
• Gene ID (NCBI): 94
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ACVRL1 (also known as ALK1) is a type I cell-surface receptor for the TGF-beta superfamily of ligands. It shares with other type I receptors a high degree of similarity in serine-threonine kinase subdomains, a glycine- and serine-rich region (called the GS domain) preceding the kinase domain, and a short C-terminal tail. ACVRL1 is highly expressed in endothelial cells and has a critical role in the control of blood vessel development and repair (PMID: 8640225). Mutations in the ACVRL1 gene are associated with hemorrhagic telangiectasia type 2.