ACY1 Rekombinanter Antikörper

ACY1 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

250846D2

Kat-Nr. : 86204-1-RR

Synonyme

aminoacylase 1, ACY 1, ACY-1, Aminoacylase-1, EC:3.5.1.14

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  WB analysis using 86204-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86204-1-RR (ACY1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 86204-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86204-1-RR against Human ACY1 were performed. The affinity constant is 0.41 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, K-562-Zellen, Neuro-2a-Zellen, NIH/3T3-Zellen, PC-12-Zellen, PC-3-Zellen, Rattenhirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86204-1-RR bindet in WB, ELISA ACY1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ACY1 fusion protein Ag1813
• Vollständiger Name: aminoacylase 1
• Berechnetes Molekulargewicht: 40.8 kDa
• GenBank-Zugangsnummer: BC014112
• Gene symbol: ACY1
• Gene ID (NCBI): 95
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Aminoacylase 1 (ACY1) is a cytosolic enzyme that is widely distributed in mammalian tissues and catalyzes the hydrolysis of acylated amino acids into amino acids and acyl groups. The main functions of ACY1 are to accelerate the hydrolysis of N-acetylated peptides, especially N-acetylated neutral aliphatic amino acids, and participate in protein synthesis and turnover through the release of free amino acids.