ADA Polyklonaler Antikörper

ADA Polyklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(9)

Rezensionen (2)

Kat-Nr. : 13328-1-AP

Synonyme

EC:3.5.4.4, ADA1

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  WB analysis of HeLa using 13328-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13328-1-AP (ADA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13328-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13328-1-AP (ADA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 13328-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13328-1-AP (ADA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of Sp2/0 using 13328-1-AP

  Sp2/0 cells were subjected to SDS PAGE followed by western blot with 13328-1-AP (ADA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 13328-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13328-1-AP (ADA Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human stomach using 13328-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13328-1-AP (ADA Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human tonsillitis using 13328-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13328-1-AP (ADA Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human tonsillitis using 13328-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13328-1-AP (ADA Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human cervical cancer using 13328-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 13328-1-AP (ADA Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human cervical cancer using 13328-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 13328-1-AP (ADA Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of mouse small intestine using 13328-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using ADA antibody (13328-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse small intestine using 13328-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using ADA antibody (13328-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HeLa-Zellen, Jurkat-Zellen, Sp2/0-Zellen
• Erfolgreiche Detektion in IHC: humanes Magengewebe, humanes Zervixkarzinomgewebe, humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maus-Dünndarmgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 8 publications below
• IHC: See 3 publications below

Produktinformation

13328-1-AP bindet in WB, IHC, IF-P, ELISA ADA und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ADA fusion protein Ag4128
• Vollständiger Name: adenosine deaminase
• Berechnetes Molekulargewicht: 363 aa, 41 kDa
• Beobachtetes Molekulargewicht: 45-47 kDa
• GenBank-Zugangsnummer: BC040226
• Gene symbol: ADA
• Gene ID (NCBI): 100
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Adenosine deaminase (ADA, EC 3.5.4.4), which has been found in plants, bacteria, and mammals including humans and others, catalyzes the hydrolysis of adenosine to yield inosine. It was demonstrated that ADA also catalyzes the hydrolysis of the derivatives of nucleoside adenosine such as 2′-deoxyadenosine. ADA is expressed on the surface of T and B cells and plays an important role in the development of immune system. (PMID: 26038697)

Publikationen

Species	Application	Title
human	WB, IHC	Front Cell Dev Biol; Thyroid Hormone-Regulated Expression of Period2 Promotes Liver Urate Production.; Authors - Xiaoting Chen
mouse	WB	Eur J Pharmacol; Adenosine kinase (ADK) inhibition with ABT-702 induces ADK protein degradation and a distinct form of sustained cardioprotection.; Authors - Gerald Wölkart
mouse	WB,IHC	Cell Death Dis; Inosine enhances tumor mitochondrial respiration by inducing Rag GTPases and nascent protein synthesis under nutrient starvation; Authors - Mei-Xin Li
human	WB	bioRxiv; The SAGA acetyltransferase module is required for the maintenance of MAF and MYC oncogenic gene expression programs in multiple myeloma; Authors - Ying-Jiun C Chen
	WB	NPJ Biofilms Microbiomes; Lactobacillus rhamnosus GG ameliorates hyperuricemia in a novel model; Authors - Yang Fu
mouse	WB	Animal Model Exp Med; The cumulative analgesic effect of repeated electroacupuncture is modulated by Adora3 in the SCDH of mice with neuropathic pain; Authors - Faisal Ayub Kiani

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Neta (Verified Customer) (07-28-2022)

antibody worked, signal slightly different than reported but overall OK.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: mouse small intestine

FH

Ryan (Verified Customer) (02-28-2019)

Tissue was fixed in PFA with NaCit antigen retrieval ph=6.

• Applications: Immunohistochemistry, Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Zebrafish brain (neurons) at 3dpf

ADA Antibody Immunohistochemistry,Immunofluorescence, validation (1:100 dilution) in Zebrafish brain (neurons) at 3dpf (Cat no:13328-1-AP)