ADA Monoklonaler Antikörper

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ADA Monoklonal Antikörper für WB, FC (Intra), ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

WB, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

2C4E9

Kat-Nr. : 67870-1-PBS

Synonyme

2C4E9, ADA1, EC:3.5.4.4

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• 

  WB analysis using 67870-1-Ig (same clone as 67870-1-PBS)

  Jurkat and MOLT-4 cells were subjected to SDS PAGE followed by western blot with 67870-1-Ig (ADA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67870-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 67870-1-Ig (same clone as 67870-1-PBS)

  WB result of ADA antibody (67870-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ADA transfected Jurkat cells. This data was developed using the same antibody clone with 67870-1-PBS in a different storage buffer formulation.

• 

  WB analysis of LNCaP using 67870-1-Ig (same clone as 67870-1-PBS)

  LNCaP cells were subjected to SDS PAGE followed by western blot with 67870-1-Ig (ADA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67870-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 67870-1-Ig (same clone as 67870-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67870-1-Ig (ADA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67870-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat spleen using 67870-1-Ig (same clone as 67870-1-PBS)

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 67870-1-Ig (ADA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67870-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rabbit spleen using 67870-1-Ig (same clone as 67870-1-PBS)

  rabbit spleen tissue were subjected to SDS PAGE followed by western blot with 67870-1-Ig (ADA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67870-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67870-1-Ig (same clone as 67870-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67870-1-Ig (ADA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67870-1-PBS in a different storage buffer formulation.

• 

  FC experiment of U2OS using 67870-1-Ig (same clone as 67870-1-PBS)

  1X10^6 U2OS cells were intracellularly stained with 0.4 ug Anti-Human ADA (67870-1-Ig, Clone:2C4E9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67870-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 67870-1-Ig

  Biolayer interferometry (BLl) kinetic assay of 67870-2-Ig against Human ADA was performed. The affinity constant is 12.8 nM.

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Geprüfte Anwendungen

Produktinformation

67870-1-PBS bindet in WB, FC (Intra), ELISA ADA und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ADA fusion protein Ag31326
• Vollständiger Name: adenosine deaminase
• Berechnetes Molekulargewicht: 363 aa, 41 kDa
• Beobachtetes Molekulargewicht: 41 kDa
• GenBank-Zugangsnummer: BC040226
• Gene symbol: ADA
• Gene ID (NCBI): 100
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.