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ADAM10 Monoklonaler Antikörper

ADAM10 Monoklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2A6B7

Kat-Nr. : 66620-1-PBS

Synonyme

ADAM 10, Disintegrin and metalloproteinase domain-containing protein 10, EC:3.4.24.81, KUZ, MADM

Filter:
  • Western Blot (WB) analysis of THP-1 cells using ADAM10 Monoclonal antibody (66620-1-Ig)

    WB analysis of THP-1 using 66620-1-Ig (same clone as 66620-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66620-1-Ig (ADAM10 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66620-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ADAM10 Monoclonal antibody (66620-1-Ig)

    WB analysis using 66620-1-Ig (same clone as 66620-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 66620-1-Ig (ADAM10 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66620-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using ADAM10 Monoclonal antibody (66620-1-Ig)

    IHC staining of human prostate cancer using 66620-1-Ig (same clone as 66620-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66620-1-Ig (ADAM10 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66620-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using ADAM10 Monoclonal antibody (66620-1-Ig)

    IHC staining of human prostate cancer using 66620-1-Ig (same clone as 66620-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66620-1-Ig (ADAM10 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66620-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

66620-1-PBS bindet in WB, IHC, Indirect ELISA ADAM10 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen ADAM10 fusion protein Ag23062
Vollständiger Name ADAM metallopeptidase domain 10
Berechnetes Molekulargewicht 748 aa, 84 kDa
Beobachtetes Molekulargewicht 90-95 kDa
GenBank-ZugangsnummerBC126253
Gene symbol ADAM10
Gene ID (NCBI) 102
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ADAM10, a member of the ADAM (a disintegrin and metalloprotease) family, is widely expressed in the brain, the spinal cord, and the visual system during development.MW of premature ADAM10 is 90 kDa and mature ADAM10 is 60-70 kDa (PMID: 24404179).