ADAM17 Polyklonaler Antikörper

ADAM17 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

Kat-Nr. : 29948-1-AP

Synonyme

ADAM 17, cSVP, Disintegrin and metalloproteinase domain-containing protein 17, EC:3.4.24.86, Snake venom-like protease

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  WB analysis using 29948-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29948-1-AP (ADAM17 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HUVEC using 29948-1-AP

  HUVEC cells were subjected to SDS PAGE followed by western blot with 29948-1-AP (ADAM17 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HUVEC-Zellen, MCF-7-Zellen, RAW 264.7-Zellen, SKOV-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IF: See 1 publications below

Produktinformation

29948-1-AP bindet in WB, IF, ELISA ADAM17 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ADAM17 fusion protein Ag32418
• Vollständiger Name: ADAM metallopeptidase domain 17
• Berechnetes Molekulargewicht: 824 aa, 93 kDa
• Beobachtetes Molekulargewicht: 90-110 kDa
• GenBank-Zugangsnummer: BC136783
• Gene symbol: ADAM17
• Gene ID (NCBI): 6868
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The ADAMs (A Disintegrin And Metalloprotease) are multidomain transmembrane proteins. One of the first ADAMs implicated in membrane shedding is ADAM-17, which is shown to release the active form of tumor necrosis factor (TNF)-a from its precursor (PMID:18238782). ADAM17 is also named as CSVP, TACE. The full length protein has 9 glycosylation sites, a signal peptide, propeptide and 2 isoforms produced by alternative splicing.The 120-kDa form of ADAM-17 is expressed more frequently and at higher levels in primary breast carcinomas compared with normal breast tissue(PMID:17438092).

Publikationen

Species	Application	Title
mouse	WB	Int J Oral Sci; Administration of Porphyromonas gingivalis in pregnant mice enhances glycolysis and histone lactylation/ADAM17 leading to cleft palate in offspring; Authors - Xige Zhao
human	IF	Mol Cell Endocrinol; Embryo-derived human chorionic gonadotropin promotes human decidualization through activating epithelial prostaglandin F2α secretion; Authors - Dan-Dan Jin
mouse	WB	Theranostics; Neutrophil-like pH-responsive pro-efferocytic nanoparticles improve neurological recovery by promoting erythrophagocytosis after intracerebral hemorrhage; Authors - Linfeng Fan
human	WB	Nat Commun; ADAM9 promotes type I interferon-mediated innate immunity during encephalomyocarditis virus infection; Authors - Lindsey E Bazzone
human	WB	Cancer Manag Res; DSG2 and c-MYC Interact to Regulate the Expression of ADAM17 and Promote the Development of Cervical Cancer; Authors - Li-Mian Song
mouse,human	WB	Arch Biochem Biophys; Maresin1 restrains chronic inflammation and Aβ production to ameliorate Alzheimer's disease via modulating ADAM10/17 and its associated neuroprotective signal pathways: A pilot study; Authors - Shuang Sun ; KD Validated