ADAM17 Monoklonaler Antikörper

ADAM17 Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

2F4C2

Kat-Nr. : 68725-1-Ig

Synonyme

ADAM 17, ADAM17, CD156b, cSVP, Snake venom like protease, TACE, TNF alpha convertase, TNF alpha converting enzyme

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  WB analysis using 68725-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68725-1-Ig (ADAM17 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 68725-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68725-1-Ig (ADAM17 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: U2OS-Zellen, A549-Zellen, HeLa-Zellen, NIH/3T3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68725-1-Ig bindet in WB, ELISA ADAM17 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ADAM17 fusion protein Ag34022
• Vollständiger Name: ADAM metallopeptidase domain 17
• Berechnetes Molekulargewicht: 824 aa, 93 kDa
• Beobachtetes Molekulargewicht: 90-110 kDa
• GenBank-Zugangsnummer: BC136783
• Gene symbol: ADAM17
• Gene ID (NCBI): 6868
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The ADAMs (A Disintegrin And Metalloprotease) are multidomain transmembrane proteins. One of the first ADAMs implicated in membrane shedding is ADAM-17, which is shown to release the active form of tumor necrosis factor (TNF)-a from its precursor (PMID:18238782). ADAM17 is also named as CSVP, TACE. The full length protein has 9 glycosylation sites, a signal peptide, propeptide and 2 isoforms produced by alternative splicing.The 120-kDa form of ADAM-17 is expressed more frequently and at higher levels in primary breast carcinomas compared with normal breast tissue(PMID:17438092).