ADAM17 Monoklonaler Antikörper

ADAM17 Monoklonal Antikörper für WB, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

WB, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2F4C2

Kat-Nr. : 68725-1-PBS

Synonyme

Snake venom-like protease, EC:3.4.24.86, Disintegrin and metalloproteinase domain-containing protein 17, cSVP, ADAM 17

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  WB analysis using 68725-1-Ig (same clone as 68725-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68725-1-Ig (ADAM17 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68725-1-PBS in a different storage buffer formulation.

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  WB analysis using 68725-1-Ig (same clone as 68725-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68725-1-Ig (ADAM17 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68725-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50392-2

  Cytometric bead array standard curve of MP50392-2, ADAM17 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68725-2-PBS. Detection antibody: 68725-1-PBS. Standard:Ag34022. Range: 0.098-100 ng/mL.

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Geprüfte Anwendungen

Produktinformation

68725-1-PBS bindet in WB, Cytometric bead array, Indirect ELISA ADAM17 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ADAM17 fusion protein Ag34022
• Vollständiger Name: ADAM metallopeptidase domain 17
• Berechnetes Molekulargewicht: 824 aa, 93 kDa
• Beobachtetes Molekulargewicht: 90-110 kDa
• GenBank-Zugangsnummer: BC136783
• Gene symbol: ADAM17
• Gene ID (NCBI): 6868
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The ADAMs (A Disintegrin And Metalloprotease) are multidomain transmembrane proteins. One of the first ADAMs implicated in membrane shedding is ADAM-17, which is shown to release the active form of tumor necrosis factor (TNF)-a from its precursor (PMID:18238782). ADAM17 is also named as CSVP, TACE. The full length protein has 9 glycosylation sites, a signal peptide, propeptide and 2 isoforms produced by alternative splicing.The 120-kDa form of ADAM-17 is expressed more frequently and at higher levels in primary breast carcinomas compared with normal breast tissue(PMID:17438092).