ADAM17 Rekombinanter Antikörper

ADAM17 Rekombinant Antikörper für WB, IHC, FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

241305A2

Kat-Nr. : 84292-4-PBS

Synonyme

241305A2, ADAM 17, cSVP, Disintegrin and metalloproteinase domain-containing protein 17, EC:3.4.24.86

---

• 

  WB analysis using 84292-4-RR (same clone as 84292-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84292-4-RR (ADAM17 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84292-4-PBS in a different storage buffer formulation.

• 

  Sandwich ELISA standard curve of MP01169-3

  Sandwich ELISA standard curve of MP01169-3, Human ADAM17 Recombinant Matched Antibody Pair - PBS only. 84292-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag32418. 84292-4-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL

• 

  Sample test of MP01169-3

  The mean ADAM17 concentration was determined to be 18.51 ng/mL in THP-1 cell extract based on a 4.4 mg/mL extract load and 13.76 ng/mL in A549 cell extract based on a 2.7 mg/mL extract load.

• 

  Sample test of MP01169-3

  Serum of six individual healthy human donors was measured. The human ADAM17 concentration of detected samples was determined to be 1,898.9 pg/mL with a range of 21.5 - 8,096.2 ng/mL.

• 

  IHC staining of human rectal cancer using 84292-4-RR (same clone as 84292-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 84292-4-RR (ADAM17 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84292-4-PBS in a different storage buffer formulation.

• 

  FC experiment of HCT 116 using 84292-4-RR (same clone as 84292-4-PBS)

  1x10^6 HCT 116 cells were intracellularly stained with 0.25 ug ADAM17 Recombinant antibody (84292-4-RR, Clone:241305A2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84292-4-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 84292-4-PBS

  Biolayer interferometry (BLl) kinetic assay of 84292-4-PBS against Human ADAM17 was performed. The affinity constant is 1.86 nM.

• 

  Affinity and Kinetic Characterization of 84292-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84292-4-RR against Human ADAM17 were performed. The affinity constant is 0.385 nM.

---

Geprüfte Anwendungen

Produktinformation

84292-4-PBS bindet in WB, IHC, FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test ADAM17 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ADAM17 fusion protein Ag32418
• Vollständiger Name: ADAM metallopeptidase domain 17
• Berechnetes Molekulargewicht: 824 aa, 93 kDa
• Beobachtetes Molekulargewicht: 100-130 kDa
• GenBank-Zugangsnummer: BC136783
• Gene symbol: ADAM17
• Gene ID (NCBI): 6868
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The ADAMs (A Disintegrin And Metalloprotease) are multidomain transmembrane proteins. One of the first ADAMs implicated in membrane shedding is ADAM-17, which is shown to release the active form of tumor necrosis factor (TNF)-a from its precursor (PMID:18238782). ADAM17 is also named as CSVP, TACE. The full length protein has 9 glycosylation sites, a signal peptide, propeptide and 2 isoforms produced by alternative splicing.The 120-kDa form of ADAM-17 is expressed more frequently and at higher levels in primary breast carcinomas compared with normal breast tissue(PMID:17438092).