ADAM2 Polyklonaler Antikörper

ADAM2 Polyklonal Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13952-1-PBS

Synonyme

---

• 

  WB analysis of mouse testis using 13952-1-AP (same clone as 13952-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 13952-1-AP (ADAM2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13952-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse testis using 13952-1-AP (same clone as 13952-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 13952-1-AP (ADAM2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13952-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse testis using 13952-1-AP (same clone as 13952-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 13952-1-AP (ADAM2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13952-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human testis using 13952-1-AP (same clone as 13952-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 13952-1-AP (ADAM2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13952-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

13952-1-PBS bindet in WB, Indirect ELISA ADAM2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ADAM2 fusion protein Ag5063
• Vollständiger Name: ADAM metallopeptidase domain 2
• Berechnetes Molekulargewicht: 82 kDa
• Beobachtetes Molekulargewicht: 50-55 kDa, 65-75 kDa
• GenBank-Zugangsnummer: BC034957
• Gene symbol: ADAM2
• Gene ID (NCBI): 2515
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The members of the ADAM (a disintegrin and metalloprotease) family are membrane-anchored multi-domain proteins that play prominent roles in male reproduction(PMID: 27341348). Fertilin, a heterodimeric protein complex composed of a(ADAM1) and b(ADAM2) subunits present on the sperm surface, has been believed to mediate adhesion and fusion between the sperm and egg plasma membranes. ADAM2-deficient mouse sperm is also defective in adhesion to and fusion with the egg membrane (PMID:16407235). It has 2 isoforms produced by alternative splicing and 5 glycosylation sites.