ADAM28 Polyklonaler Antikörper

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• KD/KO Validated

ADAM28 Polyklonal Antikörper für WB, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 22234-1-PBS

Synonyme

eMDCII, eMDC II, EC:3.4.24.-, Disintegrin and metalloproteinase domain-containing protein 28, ADAM23

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  WB analysis of mouse lung using 22234-1-AP (same clone as 22234-1-PBS)

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 22234-1-AP (ADAM28 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22234-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 22234-1-AP (same clone as 22234-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 22234-1-AP (ADAM28 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22234-1-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-13 using 22234-1-AP (same clone as 22234-1-PBS)

  PC-13 cells were subjected to SDS PAGE followed by western blot with 22234-1-AP (ADAM28 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22234-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse lung using 22234-1-AP (same clone as 22234-1-PBS)

  IP result of anti-ADAM28 (IP:22234-1-AP, 4ug; Detection:22234-1-AP 1:800) with mouse lung tissue lysate 3600ug. This data was developed using the same antibody clone with 22234-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

22234-1-PBS bindet in WB, IP, Indirect ELISA ADAM28 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ADAM28 fusion protein Ag17586
• Vollständiger Name: ADAM metallopeptidase domain 28
• Berechnetes Molekulargewicht: 775 aa, 87 kDa
• Beobachtetes Molekulargewicht: 35 kDa
• GenBank-Zugangsnummer: BC136478
• Gene symbol: ADAM28
• Gene ID (NCBI): 10863
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ADAM28 belongs to a family of cell surface and secreted glycoproteins that possess both proteolytic and adhesive properties. ADAM28 is predominantly expressed by carcinoma cells in the breast carcinoma tissues as protein bands of 42 kDa and 55/57 kDa, which correspond to the active forms of ADAM28s and ADAM28m, respectively.In human non-small cell lung carcinomas and breast carcinomas, ADAM28 is overexpressed predominantly by carcinoma cells, and the expression correlates with carcinoma cell proliferation and lymph node metastasis(PMID:19601836).