ADAM8 Polyklonaler Antikörper

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• KD/KO Validated

ADAM8 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Kat-Nr. : 23778-1-AP

Synonyme

ADAM metallopeptidase domain 8, ADAM8, ADAM8 protein, CD156, MS2

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Galerie der Validierungsdaten

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  WB analysis of BxPC-3 using 23778-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 23778-1-AP (ADAM8 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse pancreas using 23778-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 23778-1-AP (ADAM8 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 23778-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 23778-1-AP (ADAM8 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human tonsillitis using 23778-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 23778-1-AP (ADAM8 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human pancreas using 23778-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 23778-1-AP (ADAM8 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 23778-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 23778-1-AP (ADAM8 Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: BxPC-3-Zellen, Maus-Pankreasgewebe
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, humanes Pankreasgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 4 publications below
• IP: See 1 publications below

Produktinformation

23778-1-AP bindet in WB, IP, IHC, ELISA ADAM8 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ADAM8 fusion protein Ag17262
• Vollständiger Name: ADAM metallopeptidase domain 8
• Berechnetes Molekulargewicht: 824 aa, 89 kDa
• Beobachtetes Molekulargewicht: 65 kDa
• GenBank-Zugangsnummer: BC115404
• Gene symbol: ADAM8
• Gene ID (NCBI): 101
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ADAMs are a large family of transmembrane proteins that are involved in proteolysis, making them candidates for mediating the remodeling of the extracellular matrix(ECM). ADAM8, one of the members of the ADAM family, is overexpressed in various human tumors. It has been shown previously that hypoxia influences the expression of some ADAM proteins. The protein found to be expressed in two active forms(90 kDa and 60 kDa): potential shedding protein and remnant protein in the pancreatic tissues under chronic pancreatitis and pancreatic cancer(PMID:18566576). The full length protein has a signal peptide and four glycosylation sites. This antibody is specific to ADAM8.

Publikationen

Species	Application	Title
mouse	IHC	Mol Cell Neurosci; Matrix metalloprotease-mediated cleavage of neural glial-related cell adhesion molecules activates quiescent olfactory stem cells via EGFR.; Authors - Zhen-Huang Chen
mouse	WB,IHC	Exp Anim; Suppression of ADAM8 attenuates angiotensin II-induced cardiac fibrosis and endothelial-mesenchymal transition via inhibiting TGF-β1/Smad2/Smad3 pathways.; Authors - Lixia Yao ; KD Validated
mouse	WB,IP	J Healthc Eng; ADAM8 Activates NLRP3 Inflammasome to Promote Cerebral Ischemia-Reperfusion Injury.; Authors - Hongwei Lu
human	WB	Pathol Oncol Res; High ADAM8 expression is associated with poor prognosis in patients with hepatocellular carcinoma.; Authors - Yun Zhang
human	WB,IHC	Int J Mol Med; ADAM17 promotes lymph node metastasis in gastric cancer via activation of the Notch and Wnt signaling pathways.; Authors - Wei Li
mouse	WB	Nat Med; Translation control of the immune checkpoint in cancer and its therapeutic targeting.; Authors - Yichen Xu