ADAM8 Monoklonaler Antikörper

ADAM8 Monoklonal Antikörper für WB, FC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human

Anwendung

WB, FC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3A12H2

Kat-Nr. : 68113-1-PBS

Synonyme

MS2, EC:3.4.24.-, Disintegrin and metalloproteinase domain-containing protein 8, Cell surface antigen MS2, CD156a

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  WB analysis of human testis using 68113-1-Ig (same clone as 68113-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 68113-1-Ig (ADAM8 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68113-1-PBS in a different storage buffer formulation.

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  WB analysis of HaCaT using 68113-1-Ig (same clone as 68113-1-PBS)

  HaCaT cells were subjected to SDS PAGE followed by western blot with 68113-1-Ig (ADAM8 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68113-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 68113-1-Ig (same clone as 68113-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 68113-1-Ig (ADAM8 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68113-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68113-1-Ig (same clone as 68113-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68113-1-Ig (ADAM8 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68113-1-PBS in a different storage buffer formulation.

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  FC experiment of A431 using 68113-1-Ig (same clone as 68113-1-PBS)

  1x10^6 A431 cells were surface stained with 0.8 ug ADAM8 Monoclonal antibody (68113-1-Ig, Clone:3A12H2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were not fixed. This data was developed using the same antibody clone with 68113-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68113-1-PBS bindet in WB, FC, Indirect ELISA ADAM8 und zeigt Reaktivität mit Hausschwein, human

• Getestete Reaktivität: Hausschwein, human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ADAM8 fusion protein Ag17262
• Vollständiger Name: ADAM metallopeptidase domain 8
• Berechnetes Molekulargewicht: 824 aa, 89 kDa
• Beobachtetes Molekulargewicht: 90 kDa
• GenBank-Zugangsnummer: BC115404
• Gene symbol: ADAM8
• Gene ID (NCBI): 101
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ADAM8 is a type I transmembrane (TM) glycoprotein consisting of an N-terminal prodomain, followed by a metalloproteinase-, disintegrin (DIS)-, cysteine-rich-, epidermal growth factor (EGF)-like- and TM domain and a cytoplasmic tail. Expression levels of ADAM8 in normal tissue are typically low and limited to a few distinct cell types in the lymphatic organs as components of the immune system, the central nervous system, bone, and the lung. This antibody can be used to detect endogenous ADAM8 with an apparent molecular weight of 90 kDa (the calculated MW ). ADAM8 also can be detectable as a band with a molecular weight (MW) of 98 kDa, slightly larger than the calculated MW (90 kDa) (PMID:11050116).