ADAMTS12 Polyklonaler Antikörper

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• KD/KO Validated

ADAMTS12 Polyklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Kat-Nr. : 24934-1-AP

Synonyme

ADAM TS 12, ADAM TS12, ADAMTS 12, ADAMTS12, PRO4389

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Galerie der Validierungsdaten

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  WB analysis of mouse placenta using 24934-1-AP

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 24934-1-AP (ADAMTS12 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 24934-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 24934-1-AP (ADAMTS12 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IF Staining of NIH/3T3 using 24934-1-AP

  Immunofluorescent analysis of NIH/3T3 cells using 24934-1-AP (ADAMTS12 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Plazenta-Gewebe, NIH/3T3-Zellen
• Erfolgreiche Detektion in IF: NIH/3T3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 9 publications below
• IF: See 1 publications below

Produktinformation

24934-1-AP bindet in WB, IF, ELISA ADAMTS12 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ADAMTS12 fusion protein Ag20712
• Vollständiger Name: ADAM metallopeptidase with thrombospondin type 1 motif, 12
• Berechnetes Molekulargewicht: 178 kDa
• Beobachtetes Molekulargewicht: 155 kDa
• GenBank-Zugangsnummer: BC058841
• Gene symbol: ADAMTS12
• Gene ID (NCBI): 81792
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ADAMTS12(A disintegrin and metalloproteinase with thrombospondin motifs 12) is also named as AT12, ADAM-TS12, PRO4389 and belongs to the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. It may play roles in pulmonary cells during fetal development or in tumor processes through its proteolytic activity or as a molecule potentially involved in regulation of cell adhesion. ADAMTS7 and ADAMTS12 appear to be potent negative regulators of endplate cartilage development(PMID:22247065). This protein has 3 isoforms produced by alternative splicing. The full length protein has 14 glycosylation sites.

Publikationen

Species	Application	Title
human	WB	Oncotarget; Cleavage of Fibulin-2 by the aggrecanases ADAMTS-4 and ADAMTS-5 contributes to the tumorigenic potential of breast cancer cells.; Authors - Tania Fontanil
human	WB,IF	Hum Mol Genet; Epigenetic changes in reterm birth placenta suggest a role for ADAMTS genes in spontaneous preterm birth.; Authors - Sneha Mani ; KD Validated
human	WB	Cell Signal; ADAMTS12 promotes oxaliplatin chemoresistance and angiogenesis in gastric cancer through VEGF upregulation; Authors - Yingming Jiang
human	WB	Sci Rep; ADAMTS12 acts as a tumor microenvironment related cancer promoter in gastric cancer.; Authors - Yangming Hou ; KD Validated
human	WB	Int J Mol Med; Overexpression of PRDM13 inhibits glioma cells via Rho and GTP enzyme activation protein.; Authors - Linna Zhang
human	WB	Hepatobiliary Pancreat Dis Int; ADAMTS12 promotes migration and epithelial-mesenchymal transition and predicts poor prognosis for pancreatic cancer.; Authors - Rui-Zhe He ; KD Validated