ADAMTS13 Rekombinanter Antikörper

ADAMTS13 Rekombinant Antikörper für WB, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

250498E11

Kat-Nr. : 85974-3-PBS

Synonyme

ADAM TS 13, ADAM TS13, ADAMTS 13, C9orf8, TTP

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  WB analysis of human plasma using 85974-3-RR (same clone as 85974-3-PBS)

  human plasma were subjected to SDS PAGE followed by western blot with 85974-3-RR (ADAMTS13 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85974-3-PBS in a different storage buffer formulation.

• 

  Sandwich ELISA standard curve of MP02235-2

  Sandwich ELISA standard curve of MP02235-2, Human ADAMTS13 Recombinant Matched Antibody Pair - PBS only. 85974-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg2556. 85974-1-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL

• 

  Sample test of MP02235-2

  Serum of six individual healthy human donors was measured. The human ADAMTS13 concentration of detected samples was determined to be 308.14 ng/mL with a range of 133.71 - 456.88 ng/mL.

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Geprüfte Anwendungen

Produktinformation

85974-3-PBS bindet in WB, Sandwich ELISA, Indirect ELISA, Sample test ADAMTS13 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ADAMTS13 fusion protein Eg2556
• Vollständiger Name: ADAM metallopeptidase with thrombospondin type 1 motif, 13
• Berechnetes Molekulargewicht: 154 kDa
• Beobachtetes Molekulargewicht: 180 kDa
• GenBank-Zugangsnummer: NM_139025
• Gene symbol: ADAMTS13
• Gene ID (NCBI): 11093
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ADAMTS13 (a disintegrin and metalloproteinase with thrombospondin type 1 motifs, member 13) is a zinc-dependent metalloproteinase that contains multiple domains, such as the signal peptide, propeptide, metalloproteinase domain, disintegrin-like domain, and TSP-1 domain, among others. The primary function of ADAMTS13 is to specifically cleave the ultra-large multimers of von Willebrand factor (vWF), preventing excessive platelet aggregation and the formation of microvascular thrombi. Deficiency of ADAMTS13 is closely associated with thrombotic thrombocytopenic purpura (TTP), a severe disease characterized by thrombocytopenia, microvascular thrombosis, and tissue damage. Additionally, reduced ADAMTS13 activity is also linked to diseases such as myocardial infarction and stroke.