ADAMTS2 Rekombinanter Antikörper

ADAMTS2 Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

241860B8

Kat-Nr. : 84476-6-RR

Synonyme

ADAMTS-2, pNPI, PCPNI, PCINP, ADAMTS 2

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  WB analysis using 84476-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84476-6-RR (ADAMTS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 84476-6-RR

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using ADAMTS2 antibody (84476-6-RR, Clone: 241860B8 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 84476-6-RR

  Biolayer interferometry (BLl) kinetic assays of 84476-6-RR against Human ADAMTS2 were performed. The affinity constant is 0.472 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HT-1080.Zellen, PC-3-Zellen
• Erfolgreiche Detektion in IF/ICC: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84476-6-RR bindet in WB, IF/ICC, ELISA ADAMTS2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ADAMTS2 fusion protein Ag34957
• Vollständiger Name: ADAM metallopeptidase with thrombospondin type 1 motif, 2
• Berechnetes Molekulargewicht: 135 kDa
• Beobachtetes Molekulargewicht: 110~130 kDa
• GenBank-Zugangsnummer: NM_014244
• Gene symbol: ADAMTS2
• Gene ID (NCBI): 9509
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

A disintegrin and metalloproteinase with thrombospondin motifs 2 (ADAMTS-2) is a metalloproteinase that plays a key role in the processing of fibrillar procollagen precursors into mature collagen molecules by excising the amino-propeptide. ADAMTS-2 can, like the procollagen C-proteinases, be regulated by transforming growth factor-β1 (TGF-β1), with implications for mechanisms whereby this growth factor effects net increases in formation of extracellular matrix. Mutations in the ADAMTS-2 gene cause dermatosparaxis in human (dermatosparactic type of Ehlers-Danlos syndrome, also known as Ehlers-Danlos syndrome type VIIC). Human ADAMTS-2 has eight potential sites for Asn-linked glycosylation and other mammals (PMID: 16046392, 12646579).