ADAMTS4 Polyklonaler Antikörper

ADAMTS4 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (2)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(25)

Kat-Nr. : 11865-1-AP

Synonyme

A disintegrin and metalloproteinase with thrombospondin motifs 4, ADAM TS 4, ADAM TS4, ADAMTS 4, ADAM-TS 4

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  WB analysis using 11865-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11865-1-AP (ADAMTS4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 11865-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11865-1-AP (ADAMTS4 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 11865-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11865-1-AP (ADAMTS4 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human colon cancer using 11865-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11865-1-AP (ADAMTS4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 11865-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11865-1-AP (ADAMTS4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:300-1:600
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 25 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Produktinformation

11865-1-AP bindet in WB, IHC, IF, ELISA ADAMTS4 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ADAMTS4 fusion protein Ag2443
• Vollständiger Name: ADAM metallopeptidase with thrombospondin type 1 motif, 4
• Berechnetes Molekulargewicht: 837 aa, 90 kDa
• Beobachtetes Molekulargewicht: 90 kDa
• GenBank-Zugangsnummer: BC030812
• Gene symbol: ADAMTS4
• Gene ID (NCBI): 9507
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The ADAMTS (a disintegrin-like and metalloproteinase with thrombospondin motifs) is a family of extracellular metalloproteinases mediating diverse functions including matrix degradation, blood coagulation and angiogenesis. The ADAMTS family constitutes a group of proteins composed of 19 enzymes and 7 ADAMTS-like proteins. ADAMTS4 is a well-known proteoglycanase and has angiomodulatory properties. The 837 amino acid long human ADAMTS4 protein contains a long signal peptide, a prodomain, a metalloproteinase catalytic domain with zinc-binding motif, a disintegrin-like domain, a central TSR motif, followed by a cysteine-rich region with ten conserved cysteine residues, and a spacer domain. The full-length proform human ADAMTS4 (zymogen form) has been described to be 90 kDa. In extracellular matrix (ECM), ADAMTS4 undergoes further C-terminal cleavage at Lys694-Phe695 and Thr581-Phe582, respectively, to generate two other truncated forms-53 and 40 kDa (PMID:12202483).

Publikationen

Species	Application	Title
rat,human	WB	Adv Sci (Weinh); Mechanical Load-Induced Upregulation of Talin2 through Non-Canonical Deubiquitination of OTUB1 Drives Facet Joint Osteoarthritis Pathogenesis; Authors - Yizhen Huang
mouse	WB	Adv Sci (Weinh); Inhibition of Heat Shock Protein 90β by Catalpol: A Potential Therapeutic Approach for Alleviating Inflammation-Induced Cartilage Injuries in Osteoarthritis; Authors - Zhenwei Zhou
rat	WB	Acta Biomater; ROS-responsive magnesium-containing microspheres for antioxidative treatment of intervertebral disc degeneration; Authors - Tianhui Zhang
pig	WB,IHC	Bone Joint Res; Suramin attenuates intervertebral disc degeneration by inhibiting NF-κB signalling pathway.; Authors - Zi-Miao Liu
mouse	WB	Mol Med Rep; Acetyl zingerone ameliorates osteoarthritis by inhibiting chondrocyte programmed cell death; Authors - Xu Chen
human	WB	Inflammation; Simvastatin Inhibits IL-1β-Induced Apoptosis and Extracellular Matrix Degradation by Suppressing the NF-kB and MAPK Pathways in Nucleus Pulposus Cells.; Authors - Ji Tu