ADAMTS4 Rekombinanter Antikörper

ADAMTS4 Rekombinant Antikörper für WB, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

4F9

Kat-Nr. : 82744-2-PBS

Synonyme

ADAM-TS 4, ADAMTS 4, ADAM TS4, ADAM TS 4, A disintegrin and metalloproteinase with thrombospondin motifs 4

---

• 

  WB analysis using 82744-2-RR (same clone as 82744-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82744-2-RR (ADAMTS4 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82744-2-PBS in a different storage buffer formulation.

• 

  FC experiment of U2OS using 82744-2-RR (same clone as 82744-2-PBS)

  1x10^6 U2OS cells were intracellularly stained with 0.25 ug ADAMTS4 Recombinant antibody (82744-2-RR, Clone:4F9) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 82744-2-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 82744-2-RR

  Biolayer interferometry (BLl) kinetic assays of 82744-2-RR against Human ADAMTS4 were performed. The affinity constant is 0.632 nM.

---

Geprüfte Anwendungen

Produktinformation

82744-2-PBS bindet in WB, FC (Intra), Indirect ELISA ADAMTS4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ADAMTS4 fusion protein Ag2443
• Vollständiger Name: ADAM metallopeptidase with thrombospondin type 1 motif, 4
• Berechnetes Molekulargewicht: 837 aa, 90 kDa
• Beobachtetes Molekulargewicht: 90 kDa
• GenBank-Zugangsnummer: BC030812
• Gene symbol: ADAMTS4
• Gene ID (NCBI): 9507
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The ADAMTS (a disintegrin-like and metalloproteinase with thrombospondin motifs) is a family of extracellular metalloproteinases mediating diverse functions including matrix degradation, blood coagulation and angiogenesis. The ADAMTS family constitutes a group of proteins composed of 19 enzymes and 7 ADAMTS-like proteins. ADAMTS4 is a well-known proteoglycanase and has angiomodulatory properties. The 837 amino acid long human ADAMTS4 protein contains a long signal peptide, a prodomain, a metalloproteinase catalytic domain with zinc-binding motif, a disintegrin-like domain, a central TSR motif, followed by a cysteine-rich region with ten conserved cysteine residues, and a spacer domain. The full-length proform human ADAMTS4 (zymogen form) has been described to be 90 kDa. In extracellular matrix (ECM), ADAMTS4 undergoes further C-terminal cleavage at Lys694-Phe695 and Thr581-Phe582, respectively, to generate two other truncated forms-53 and 40 kDa (PMID:12202483).