ADAMTSL4 Polyklonaler Antikörper

ADAMTSL4 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 15304-1-AP

Synonyme

ADAMTS like 4, ADAMTS like protein 4, ADAMTSL 4, ADAMTSL4, TSRC1, UNQ2803/PRO34012

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 15304-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15304-1-AP (ADAMTSL4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 15304-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 15304-1-AP (ADAMTSL4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 15304-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 15304-1-AP (ADAMTSL4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 15304-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15304-1-AP (ADAMTSL4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 15304-1-AP

  IP Result of anti-ADAMTSL4 (IP:15304-1-AP, 4ug; Detection:15304-1-AP 1:500) with mouse brain tissue lysate 3440ug.

• 

  IHC staining of mouse skeletal muscle using 15304-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 15304-1-AP (ADAMTSL4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 15304-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 15304-1-AP (ADAMTSL4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 15304-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15304-1-AP (ADAMTSL4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 15304-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15304-1-AP (ADAMTSL4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skeletal muscle using 15304-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 15304-1-AP (ADAMTSL4 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 15304-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 15304-1-AP (ADAMTSL4 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon cancer using 15304-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15304-1-AP (ADAMTSL4 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 15304-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15304-1-AP (ADAMTSL4 Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, humanes Lebergewebe, Mauslungengewebe, Maus-Skelettmuskelgewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: Maus-Skelettmuskelgewebe, humanes Kolonkarzinomgewebe, humanes Lebergewebe, humanes Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

15304-1-AP bindet in WB, IP, IHC, IF, ELISA ADAMTSL4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ADAMTSL4 fusion protein Ag7781
• Vollständiger Name: ADAMTS-like 4
• Berechnetes Molekulargewicht: 117 kDa
• Beobachtetes Molekulargewicht: 65-70 kDa
• GenBank-Zugangsnummer: BC027478
• Gene symbol: ADAMTSL4
• Gene ID (NCBI): 54507
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB,IP,IF	Matrix Biol; Interactions between lysyl oxidases and ADAMTS proteins suggest a novel crosstalk between two extracellular matrix families.; Authors - Rohtem Aviram
human	IHC, WB	Front Mol Biosci; An Extracellular Matrix-Based Signature Associated With Immune Microenvironment Predicts the Prognosis and Therapeutic Responses of Patients With Oesophageal Squamous Cell Carcinoma.; Authors - Hongpan Zhang
human	WB	Oncotarget; Correlation of five secretory proteins with the nasopharyngeal carcinoma metastasis and the clinical applications.; Authors - Ya Tao
rat	IHC	Int J Nanomedicine; Pioglitazone-Loaded Cartilage-Targeted Nanomicelles (Pio@C-HA-DOs) for Osteoarthritis Treatment; Authors - Junyan Chen

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