ADAR1 Polyklonaler Antikörper

ADAR1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(25)

Kat-Nr. : 14330-1-AP

Synonyme

ADAR, 136 kDa double-stranded RNA-binding protein, DSH, DRADA, Double-stranded RNA-specific adenosine deaminase

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  WB analysis of HeLa using 14330-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14330-1-AP (ADAR1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 14330-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14330-1-AP (ADAR1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 14330-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14330-1-AP (ADAR1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 14330-1-AP

  IP result of anti-ADAR1 (IP:14330-1-AP, 4ug; Detection:14330-1-AP 1:800) with HepG2 cells lysate 1320 ug.

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  IHC staining of human gliomas using 14330-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14330-1-AP (ADAR1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 14330-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14330-1-AP (ADAR1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 14330-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 14330-1-AP (ADAR1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 14330-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 14330-1-AP (ADAR1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 14330-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14330-1-AP (ADAR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 14330-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14330-1-AP (ADAR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 14330-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 14330-1-AP (ADAR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 14330-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 14330-1-AP (ADAR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 14330-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14330-1-AP (ADAR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 14330-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14330-1-AP (ADAR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 14330-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ADAR1 antibody (14330-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A431-Zellen, HepG2-Zellen, Jurkat-Zellen
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe, humanes Kolonkarzinomgewebe, humanes Magenkrebsgewebe, Maushirngewebe, Maus-Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 20 publications below
• IHC: See 6 publications below
• IF: See 8 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below

Produktinformation

14330-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA ADAR1 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ADAR1 fusion protein Ag5609
• Vollständiger Name: adenosine deaminase, RNA-specific
• Berechnetes Molekulargewicht: 136 kDa
• Beobachtetes Molekulargewicht: 110 kDa
• GenBank-Zugangsnummer: BC038227
• Gene symbol: ADAR1
• Gene ID (NCBI): 103
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ADAR1 is also named as ADAR1, DSRAD, G1P1, IFI4. It convert selected adenosine residues into inosine in substrate RNAs containing a relatively short dsRNA region(PMID:15556947). The human ADAR1 gene specifies two size forms of RNA-specific adenosine deaminase, an IFN inducible ∼150 kDa protein and a constitutively expressed N-terminally truncated ∼110 kDa protein, encoded by transcripts with alternative exon 1 structures that initiate from different promoters(PMID:11111054). It has 5 isoforms produced by alternative promoter usage and alternative splicing. Defects in ADAR are a cause of dyschromatosis symmetrical hereditaria (DSH).ADAR1 can form respective homodimers, and this association is essential for its enzymatic activities(PMID:17428802).

Publikationen

Species	Application	Title
	WB,IF	Adv Sci (Weinh); Primate-Specific DAZ Regulates Translation of Cell Proliferation-Related mRNAs and is Essential for Maintenance of Spermatogonia; Authors - Ningjing Ou
human	WB	Dev Cell; Genome-wide analysis of pseudogenes reveals HBBP1's human-specific essentiality in erythropoiesis and implication in β-thalassemia.; Authors - Yanni Ma
human	WB	Neurobiol Stress; Roles of miR-432 and circ_0000418 in mediating the anti-depressant action of ADAR1.; Authors - Xiaonan Zhang
human	WB,CoIP	J Biol Chem; ADAR promotes USP38 auto-deubiquitylation and stabilization in an RNA editing-independent manner in Esophageal Squamous Cell Carcinoma; Authors - Qingyong Hu
human	IHC	Am J Cancer Res; The aberrant expression of ADAR1 promotes resistance to BET inhibitors in pancreatic cancer by stabilizing c-Myc.; Authors - Yan Sun
human	WB	Toxicol Appl Pharmacol; The anti-cancer drug candidate CBL0137 induced necroptosis via forming left-handed Z-DNA and its binding protein ZBP1 in liver cells; Authors - Jun Li