ADAR1 Polyklonaler Antikörper

ADAR1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14330-1-PBS

Synonyme

ADAR, 136 kDa double-stranded RNA-binding protein, DSH, DRADA, Double-stranded RNA-specific adenosine deaminase

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  WB analysis of HeLa using 14330-1-AP (same clone as 14330-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 14330-1-AP (ADAR1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14330-1-PBS in a different storage buffer formulation.

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  WB analysis using 14330-1-AP (same clone as 14330-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14330-1-AP (ADAR1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14330-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 14330-1-AP (same clone as 14330-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14330-1-AP (ADAR1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14330-1-PBS in a different storage buffer formulation.

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  IP experiment of HepG2 using 14330-1-AP (same clone as 14330-1-PBS)

  IP result of anti-ADAR1 (IP:14330-1-AP, 4ug; Detection:14330-1-AP 1:800) with HepG2 cells lysate 1320 ug. This data was developed using the same antibody clone with 14330-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 14330-1-AP (same clone as 14330-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14330-1-AP (ADAR1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14330-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 14330-1-AP (same clone as 14330-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14330-1-AP (ADAR1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14330-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 14330-1-AP (same clone as 14330-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 14330-1-AP (ADAR1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14330-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 14330-1-AP (same clone as 14330-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 14330-1-AP (ADAR1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14330-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 14330-1-AP (same clone as 14330-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14330-1-AP (ADAR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14330-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 14330-1-AP (same clone as 14330-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14330-1-AP (ADAR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14330-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 14330-1-AP (same clone as 14330-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 14330-1-AP (ADAR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14330-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 14330-1-AP (same clone as 14330-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 14330-1-AP (ADAR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14330-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 14330-1-AP (same clone as 14330-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14330-1-AP (ADAR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14330-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 14330-1-AP (same clone as 14330-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14330-1-AP (ADAR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14330-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 14330-1-AP (same clone as 14330-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ADAR1 antibody (14330-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 14330-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

14330-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA ADAR1 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ADAR1 fusion protein Ag5609
• Vollständiger Name: adenosine deaminase, RNA-specific
• Berechnetes Molekulargewicht: 136 kDa
• Beobachtetes Molekulargewicht: 110 kDa
• GenBank-Zugangsnummer: BC038227
• Gene symbol: ADAR1
• Gene ID (NCBI): 103
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ADAR1 is also named as ADAR1, DSRAD, G1P1, IFI4. It convert selected adenosine residues into inosine in substrate RNAs containing a relatively short dsRNA region(PMID:15556947). The human ADAR1 gene specifies two size forms of RNA-specific adenosine deaminase, an IFN inducible ∼150 kDa protein and a constitutively expressed N-terminally truncated ∼110 kDa protein, encoded by transcripts with alternative exon 1 structures that initiate from different promoters(PMID:11111054). It has 5 isoforms produced by alternative promoter usage and alternative splicing. Defects in ADAR are a cause of dyschromatosis symmetrical hereditaria (DSH).ADAR1 can form respective homodimers, and this association is essential for its enzymatic activities(PMID:17428802).