ADARB1 Polyklonaler Antikörper

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• KD/KO Validated

ADARB1 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, IP, RIP, ELISA

Konjugation

Unkonjugiert

Publikationen(16)

Kat-Nr. : 22248-1-AP

Synonyme

RED1, ADAR2, RNA-editing deaminase 1, EC:3.5.4.37, Double-stranded RNA-specific editase 1

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  WB analysis using 22248-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22248-1-AP (ADARB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 22248-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22248-1-AP (ADARB1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 22248-1-AP

  IP result of anti-ADARB1 (IP:22248-1-AP, 4ug; Detection:22248-1-AP 1:1000) with HeLa cells lysate 1080 ug.

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  IHC staining of mouse brain using 22248-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22248-1-AP (ADARB1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 22248-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22248-1-AP (ADARB1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, 4T1-Zellen, C2C12-Zellen, HeLa-Zellen, HepG2-Zellen, NIH/3T3-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 12 publications below
• IHC: See 3 publications below
• IF: See 2 publications below
• RIP: See 2 publications below

Produktinformation

22248-1-AP bindet in WB, IHC, IF, IP, RIP, ELISA ADARB1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ADARB1 fusion protein Ag17743
• Vollständiger Name: adenosine deaminase, RNA-specific, B1 (RED1 homolog rat)
• Berechnetes Molekulargewicht: 741 aa, 81 kDa
• Beobachtetes Molekulargewicht: 74-86 kDa
• GenBank-Zugangsnummer: BC065545
• Gene symbol: ADARB1
• Gene ID (NCBI): 104
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ADARB1(Double-stranded RNA-specific editase 1) is also named as ADAR2, DRADA2, RED1 and belongs to the ADAR family. It catalyses the deamination of adenosine to inosine at the GluR2 Q/R site in the pre-mRNA encoding the critical subunit of AMPA receptors. This protein has some isoforms with the molecular weight from 77 kDa to 81 kDa and 7 kDa, but it can be detected a very strong band at 50 kDa in addition to a predicted band at 75 kDa as the result of western blot, while ADARB1 has several alternative splice variants (PMID:23103828).

Publikationen

Species	Application	Title
human	WB	Nature; Breakage of cytoplasmic chromosomes by pathological DNA base excision repair.; Authors - Shangming Tang
human	WB,RIP	Basic Res Cardiol; Reduction of A-to-I RNA editing in the failing human heart regulates formation of circular RNAs.; Authors - Karoline E Kokot
mouse	IF	Sci Rep; Functional interaction between FUS and SMN underlies SMA-like splicing changes in wild-type hFUS mice.; Authors - Alessia Mirra
mouse	WB	FASEB J; Lung fibrosis is induced in ADAR2 overexpressing mice via HuR-induced CTGF signaling.; Authors - Ramani Soundararajan ; KO Validated
human	WB	J Cell Mol Med; Lipopolysaccharide enhances ADAR2 which drives Hirschsprung's disease by impairing miR-142-3p biogenesis.; Authors - Lei Peng
human	WB	Front Pharmacol; Aberrant Expression of ADARB1 Facilitates Temozolomide Chemoresistance and Immune Infiltration in Glioblastoma.; Authors - Can Lu