ADC Monoklonaler Antikörper

ADC Monoklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

CloneNo.

1A2B11

Kat-Nr. : 67884-1-Ig

Synonyme

ADC, Antizyme inhibitor 2, ARGDC, arginine decarboxylase, AZI2, AZIN2, KIAA1945, ODC p, ODC paralogue, ODC1L, ODCP

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Galerie der Validierungsdaten

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  WB analysis of HEK-293T using 67884-1-Ig

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 67884-1-Ig (ADC antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 67884-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67884-1-Ig (ADC antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67884-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67884-1-Ig (ADC antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 67884-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 67884-1-Ig (ADC antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 67884-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67884-1-Ig (ADC antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse cerebellum using 67884-1-Ig

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 67884-1-Ig (ADC antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 67884-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 67884-1-Ig (ADC antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 67884-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using ADC antibody (67884-1-Ig, Clone: 1A2B11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293T-Zellen, Hausschwein-Hirngewebe, HEK-293-Zellen, HeLa-Zellen, Maus-Cerebellum-Gewebe, Maushirngewebe, Ratten-Cerebellum-Gewebe
• Erfolgreiche Detektion in IF: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67884-1-Ig bindet in WB, IF, ELISA ADC und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ADC fusion protein Ag31049
• Vollständiger Name: arginine decarboxylase
• Berechnetes Molekulargewicht: 460 aa, 50 kDa
• Beobachtetes Molekulargewicht: 48-50 kDa
• GenBank-Zugangsnummer: BC028128
• Gene symbol: ADC
• Gene ID (NCBI): 113451
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Antizyme inhibitor 2 (AZIN2) is a member of the antizyme inhibitor family, witch is able to bind to AZs and inhibit their action on both ODC activity and polyamine uptake. AZIN2 is predominantly expressed in the brain and testis and localized in the endoplasmic reticulum-golgi intermediate compartment. Available data suggest its role in cell growth, spermiogenesis, vesicular trafficking and secretion. Accumulation of azin2 has also been observed in brains of patients with Alzheimer's disease (PMID: 19832840) . AZIN2 has 5 described insoforms produced by alternative splicing. The molecular weight of AZIN2 is about 50 kDa.