ADC Monoklonaler Antikörper

ADC Monoklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1A2B11

Kat-Nr. : 67884-1-PBS

Synonyme

ADC, Antizyme inhibitor 2, ARGDC, arginine decarboxylase, AZI2, AZIN2, KIAA1945, ODC p, ODC paralogue, ODC1L, ODCP

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  WB analysis of HEK-293T using 67884-1-Ig (same clone as 67884-1-PBS)

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 67884-1-Ig (ADC antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67884-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67884-1-Ig (same clone as 67884-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67884-1-Ig (ADC antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67884-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67884-1-Ig (same clone as 67884-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67884-1-Ig (ADC antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67884-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 67884-1-Ig (same clone as 67884-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 67884-1-Ig (ADC antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67884-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67884-1-Ig (same clone as 67884-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 67884-1-Ig (ADC antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67884-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse cerebellum using 67884-1-Ig (same clone as 67884-1-PBS)

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 67884-1-Ig (ADC antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67884-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 67884-1-Ig (same clone as 67884-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 67884-1-Ig (ADC antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67884-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 67884-1-Ig (same clone as 67884-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using ADC antibody (67884-1-Ig, Clone: 1A2B11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67884-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67884-1-PBS bindet in WB, IF/ICC, Indirect ELISA ADC und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ADC fusion protein Ag31049
• Vollständiger Name: arginine decarboxylase
• Berechnetes Molekulargewicht: 460 aa, 50 kDa
• Beobachtetes Molekulargewicht: 48-50 kDa
• GenBank-Zugangsnummer: BC028128
• Gene symbol: ADC
• Gene ID (NCBI): 113451
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Antizyme inhibitor 2 (AZIN2) is a member of the antizyme inhibitor family, witch is able to bind to AZs and inhibit their action on both ODC activity and polyamine uptake. AZIN2 is predominantly expressed in the brain and testis and localized in the endoplasmic reticulum-golgi intermediate compartment. Available data suggest its role in cell growth, spermiogenesis, vesicular trafficking and secretion. Accumulation of azin2 has also been observed in brains of patients with Alzheimer's disease (PMID: 19832840) . AZIN2 has 5 described insoforms produced by alternative splicing. The molecular weight of AZIN2 is about 50 kDa.