ADC Monoklonaler Antikörper

ADC Monoklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

IF, FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1A2B11

Kat-Nr. : CL488-67884

Synonyme

ADC, ARGDC, arginine decarboxylase, AZI2, KIAA1945, ODC p, ODC paralogue, ODC1L, ODCP

---

Galerie der Validierungsdaten

• 

  IF Staining of A431 using CL488-67884

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CoraLite® Plus 488 ADC antibody (CL488-67884, Clone: 1A2B11 ) at dilution of 1:200.

• 

  FC experiment of A431 using CL488-67884

  1X10^6 A431 cells were intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human ADC (CL488-67884, Clone:1A2B11) (red), or 0.2 ug Mouse IgG1 Isotype Control (CL488-66360, Clone: T1F8D3F10) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: A431-Zellen
• Erfolgreiche Detektion in FC: A431-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-67884 bindet in IF, FC (Intra) ADC und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ADC fusion protein Ag31049
• Vollständiger Name: arginine decarboxylase
• Berechnetes Molekulargewicht: 460 aa, 50 kDa
• Beobachtetes Molekulargewicht: 48-50 kDa
• GenBank-Zugangsnummer: BC028128
• Gene symbol: ADC
• Gene ID (NCBI): 113451
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 488 nm / 515 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Antizyme inhibitor 2 (AZIN2) is a member of the antizyme inhibitor family, witch is able to bind to AZs and inhibit their action on both ODC activity and polyamine uptake. AZIN2 is predominantly expressed in the brain and testis and localized in the endoplasmic reticulum-golgi intermediate compartment. Available data suggest its role in cell growth, spermiogenesis, vesicular trafficking and secretion. Accumulation of azin2 has also been observed in brains of patients with Alzheimer's disease (PMID: 19832840) . AZIN2 has 5 described insoforms produced by alternative splicing. The molecular weight of AZIN2 is about 50 kDa.

Verwandte Produkte

Foxp3 / Transcription Factor Staining Buffer Kit

PF00011