ADCY6 Polyklonaler Antikörper

ADCY6 Polyklonal Antikörper für IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Rezensionen (1)

Kat-Nr. : 14616-1-AP

Synonyme

AC6, ADCY6, adenylate cyclase 6, Adenylate cyclase type 6, Adenylate cyclase type VI, Adenylyl cyclase 6, ATP pyrophosphate lyase 6, KIAA0422

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Galerie der Validierungsdaten

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  WB analysis of human brain using 14616-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14616-1-AP (ADCY6 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 14616-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14616-1-AP (ADCY6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 14616-1-AP

  IP Result of anti-ADCY6 (IP:14616-1-AP, 4ug; Detection:14616-1-AP 1:300) with mouse brain tissue lysate 4000ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hirngewebe, Maushirngewebe
• Erfolgreiche IP: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below

Produktinformation

14616-1-AP bindet in WB, IP, ELISA ADCY6 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ADCY6 fusion protein Ag6218
• Vollständiger Name: adenylate cyclase 6
• Berechnetes Molekulargewicht: 131 kDa
• Beobachtetes Molekulargewicht: 131 kDa
• GenBank-Zugangsnummer: BC064923
• Gene symbol: ADCY6
• Gene ID (NCBI): 112
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	Nat Commun; Deficiency of WTAP in hepatocytes induces lipoatrophy and non-alcoholic steatohepatitis (NASH); Authors - Xinzhi Li
mouse	WB	Mol Metab; Purβ promotes hepatic glucose production by increasing Adcy6 transcription.; Authors - Linna Jia
human	WB	Mol Pharmacol; Activity of adenylyl cyclase type 6 is suppressed by direct binding of the cytoskeletal protein 4.1G.; Authors - Masaki Saito
human	WB	Pathol Oncol Res; Knockdown of GPSM1 Inhibits the Proliferation and Promotes the Apoptosis of B-Cell Acute Lymphoblastic Leukemia Cells by Suppressing the ADCY6-RAPGEF3-JNK Signaling Pathway.; Authors - Ye Zhang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Charlotte (Verified Customer) (07-27-2022)

1 and 2 : WT starved and ShhN stimulated. 3 to 6 are ADC6 KO cells with 2 different gRNAs. 3/4 is one gRNA and 5/6 is another one. 3 and 5 are starved while 4 and 6 are ShhN stimulated

• Applications: Western Blot
• Primary Antibody Dilution: 1/500
• Cell Tissue Type: NIH-3T3

ADCY6 Antibody Western Blot validation (1/500 dilution) in NIH-3T3 (Cat no:14616-1-AP)