ADH1B Monoklonaler Antikörper

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• KD/KO Validated

ADH1B Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1F1B4

Kat-Nr. : 66939-1-PBS

Synonyme

ADH1B, Alcohol dehydrogenase 1B, DKFZp686C06125

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  WB analysis using 66939-1-Ig (same clone as 66939-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66939-1-Ig (ADH1B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66939-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 66939-1-Ig (same clone as 66939-1-PBS)

  WB result of ADH1B antibody (66939-1-Ig; 1:50000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ADH1B transfected HepG2 cells. This data was developed using the same antibody clone with 66939-1-PBS in a different storage buffer formulation.

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  WB analysis of pig liver using 66939-1-Ig (same clone as 66939-1-PBS)

  pig liver tissue were subjected to SDS PAGE followed by western blot with 66939-1-Ig (ADH1B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66939-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat liver using 66939-1-Ig (same clone as 66939-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 66939-1-Ig (ADH1B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66939-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66939-1-Ig (same clone as 66939-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66939-1-Ig (ADH1B antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66939-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66939-1-Ig (same clone as 66939-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66939-1-Ig (ADH1B antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66939-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66939-1-Ig (same clone as 66939-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ADH1B antibody (66939-1-Ig, Clone: 1F1B4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66939-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66939-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA ADH1B und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ADH1B fusion protein Ag10630
• Vollständiger Name: alcohol dehydrogenase 1B (class I), beta polypeptide
• Berechnetes Molekulargewicht: 375 aa, 40 kDa
• Beobachtetes Molekulargewicht: 35-40 kDa
• GenBank-Zugangsnummer: BC033009
• Gene symbol: ADH1B
• Gene ID (NCBI): 125
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The ADH1B gene encodes the beta subunit of class I alcohol dehydrogenase (ADH), an enzyme that catalyzes the rate-limiting step for ethanol metabolism: the oxidation of alcohol to acetaldehyde. Class 1 ADH is a homo- or heterodimeric molecule, formed by the association of 3 types of class I ADH subunits, alpha (ADH1A), beta, and gamma(ADH1C). ADH1B is also named as ADH2 and belongs to the zinc-containing alcohol dehydrogenase family. This protein can exsit as a homodimer(PMID:19365573).