ADH7 Polyklonaler Antikörper

ADH7 Polyklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 23425-1-AP

Synonyme

ADH 4, ADH7, Gastric alcohol dehydrogenase, Retinol dehydrogenase

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 23425-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 23425-1-AP (ADH7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 23425-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23425-1-AP (ADH7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 23425-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 23425-1-AP (ADH7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 23425-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver slide using 23425-1-AP (ADH7 Antibody) at dilution of 1:50.

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  IHC staining of human liver using 23425-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver slide using 23425-1-AP (ADH7 Antibody) at dilution of 1:50.

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  IF Staining of HepG2 using 23425-1-AP

  Immunofluorescent analysis of HepG2 cells, using ADH7 antibody 23425-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A549-Zellen, HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

23425-1-AP bindet in WB, IHC, IF, ELISA ADH7 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ADH7 fusion protein Ag19340
• Vollständiger Name: alcohol dehydrogenase 7 (class IV), mu or sigma polypeptide
• Berechnetes Molekulargewicht: 386 aa, 41 kDa
• Beobachtetes Molekulargewicht: 45-47 kDa
• GenBank-Zugangsnummer: BC131512
• Gene symbol: ADH7
• Gene ID (NCBI): 131
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ADH7(Alcohol dehydrogenase class 4 mu/sigma chain) is also named as gastric alcohol dehydrogenase, retinol dehydrogenase and belongs to the zinc-containing alcohol dehydrogenase family. The ubiquitous expression pattern of ADH7 during embryogenesis has led to the notion that the enzymatic conversion of retinol to retinal occurs more or less uniformly and plays no role in the spatial or temporal regulation of RA synthesis during embryonic development(PMID:17473173). It has 2 isoforms produced by alternative splicing and can exsit as a homodimer(PMID:18479436).

Publikationen

Species	Application	Title
mouse	WB	Aging (Albany NY); Mettl3-mediated m6A modification plays a role in lipid metabolism disorders and progressive liver damage in mice by regulating lipid metabolism-related gene expression; Authors - Guanqi Dai