Adiponectin Polyklonaler Antikörper

Adiponectin Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(28)

Kat-Nr. : 21613-1-AP

Synonyme

ADIPOQ, apM-1, ADIPQTL1, Adipocyte, C1q and collagen domain-containing protein, Adipocyte complement-related 30 kDa protein



Geprüfte Anwendungen

Erfolgreiche Detektion in WBhuman plasma, 3T3-L1-Zellen, NIH/3T3-Zellen
Erfolgreiche Detektion in IHCrat brown adipose tissue, humanes Plazenta-Gewebe, humanes Prostatakarzinomgewebe, humanes Hautgewebe, braunes Mausfettgewebe, Maus-Skelettmuskelgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICC3T3-L1-Zellen, NIH/3T3-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:2000
Immunhistochemie (IHC)IHC : 1:200-1:800
Immunfluoreszenz (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

21613-1-AP bindet in WB, IHC, IF/ICC, ELISA Adiponectin und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Maus, Ratte, Ziege
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Adiponectin fusion protein Ag16304
Vollständiger Name adiponectin, C1Q and collagen domain containing
Berechnetes Molekulargewicht 244 aa, 26 kDa
Beobachtetes Molekulargewicht 29 kDa
GenBank-ZugangsnummerBC096308
Gene symbol Adiponectin
Gene ID (NCBI) 9370
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Adiponectin (AdipoQ), an adipocyte-derived hormone, is one of the most abundant adipokines in the blood circulation. Adiponectin modulates a number of metabolic processes, including improving INS sensitivity and anti-inflammatory activity. The role of AdipoQ in reproduction is not yet fully understood, but the expression of AdipoQ in reproductive tissues has been observed in various animals and humans, including chicken testis, bovine ovary, and human placenta. Adiponectin exerts its effects by activating a range of different signaling molecules via binding to two transmembrane AdipoQ receptors, AdipoR1 and AdipoR2. AdipoR1 is expressed primarily in the skeletal muscle, whereas AdipoR2 is predominantly expressed in the liver. AdipoQ May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for Adiponectin antibody 21613-1-APProtokoll herunterladen
IHC protocol for Adiponectin antibody 21613-1-APProtokoll herunterladenl
IF protocol for Adiponectin antibody 21613-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
mouseIF

Diabetes

PRMT4 Facilitates White Adipose Tissue Browning and Thermogenesis by Methylating PPARγ

Authors - Yi Zhong
humanWB

Int J Mol Sci

Piperine Improves Lipid Dysregulation by Modulating Circadian Genes Bmal1 and Clock in HepG2 Cells.

Authors - Weiyun Zhang
mouseWB

Respir Res

Isthmin-1 attenuates allergic Asthma by stimulating adiponectin expression and alveolar macrophage efferocytosis in mice

Authors - Jong Huat Tee
mouseWB

J Nutr Biochem

All-trans retinoic acid induces lipophagy through the activation of the AMPK-Beclin1 signaling pathway and reduces Rubicon expression in adipocytes

Authors - Yuki Mori
humanWB, IF

Sci China Life Sci

Compression loading of osteoclasts attenuated microRNA-146a-5p expression, which promotes angiogenesis by targeting adiponectin.

Authors - Yue Wang
mouseWB

Dig Dis Sci

Adiponectin Alleviates Intestinal Fibrosis by Enhancing AMP-Activated Protein Kinase Phosphorylation.

Authors - Minghao Xie