ADK Monoklonaler Antikörper

ADK Monoklonal Antikörper für WB, IHC, IF-P, FC (Intra), Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1G1A5

Kat-Nr. : 66929-1-PBS

Synonyme

EC:2.7.1.20, AK, adenosine kinase, Adenosine 5'-phosphotransferase, 1G1A5

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  WB analysis using 66929-1-Ig (same clone as 66929-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66929-1-Ig (ADK antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66929-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66929-1-Ig (same clone as 66929-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66929-1-Ig (ADK antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66929-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66929-1-Ig (same clone as 66929-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66929-1-Ig (ADK antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66929-1-PBS in a different storage buffer formulation.

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  IF Staining of human liver cancer using 66929-1-Ig (same clone as 66929-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using ADK antibody (66929-1-Ig, Clone: 1G1A5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66929-1-PBS in a different storage buffer formulation.

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  IF Staining of human breast cancer using 66929-1-Ig (same clone as 66929-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using ADK antibody (66929-1-Ig, Clone: 1G1A5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66929-1-PBS in a different storage buffer formulation.

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  FC experiment of NIH/3T3 using 66929-1-Ig (same clone as 66929-1-PBS)

  1X10^6 NIH/3T3 cells were intracellularly stained with 0.4 ug Anti-Human ADK (66929-1-Ig, Clone:1G1A5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66929-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66929-1-PBS bindet in WB, IHC, IF-P, FC (Intra), Indirect ELISA ADK und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ADK fusion protein Ag7466
• Vollständiger Name: adenosine kinase
• Berechnetes Molekulargewicht: 41 kDa
• Beobachtetes Molekulargewicht: 40-46 kDa
• GenBank-Zugangsnummer: BC003568
• Gene symbol: ADK
• Gene ID (NCBI): 132
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.