Adenosine A1 Receptor Polyklonaler Antikörper

Adenosine A1 Receptor Polyklonal Antikörper für WB, IHC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 20332-1-AP

Synonyme

Adenosine receptor A1, ADORA1, RDC7

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  WB analysis of mouse eye using 20332-1-AP

  mouse eye tissue were subjected to SDS PAGE followed by western blot with 20332-1-AP (Adenosine A1 Receptor antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 20332-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20332-1-AP (Adenosine A1 Receptor antibody at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 20332-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20332-1-AP (Adenosine A1 Receptor antibody at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 20332-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 20332-1-AP (Adenosine A1 Receptor antibody at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 20332-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 20332-1-AP (Adenosine A1 Receptor antibody at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of SH-SY5Y using 20332-1-AP

  1x10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug Adenosine A1 Receptor Polyclonal antibody (20332-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Augengewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe, Maus-Cerebellum-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC (Intra): SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

20332-1-AP bindet in WB, IHC, IF, FC (Intra), ELISA Adenosine A1 Receptor und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Adenosine A1 Receptor fusion protein Ag14092
• Vollständiger Name: adenosine A1 receptor
• Berechnetes Molekulargewicht: 326 aa, 37 kDa
• Beobachtetes Molekulargewicht: 39 kDa
• GenBank-Zugangsnummer: BC026340
• Gene symbol: Adenosine A1 Receptor
• Gene ID (NCBI): 134
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ADORA1, also named as RDC7, is a receptor for adenosine. It is mediated by G proteins which inhibit adenylyl cyclase. Adenosine is an important mediator of ethanol intoxication and exerts some of its effects via ADORA1 in the central nervous system.

Publikationen

Species	Application	Title
mouse	IHC	Cell Biol Toxicol; Adenosine kinase protects against acetaminophen-induced acute liver injury by activating autophagy in hepatocytes; Authors - Chuanxin Zhang
human	FC (Intra)	PLoS One; Discovery of a potent, selective, and tumor-suppressing antibody antagonist of adenosine A2A receptor; Authors - Linya Wang
mouse	WB,IF	J Orthop Translat; CD73 alleviates osteoarthritis by maintaining anabolism and suppressing catabolism of chondrocytes extracellular matrix; Authors - Hu Guo
human	WB	Am J Physiol Cell Physiol; Abnormal purine metabolism in nasal epithelial cells affects allergic rhinitis by regulating Th17/Treg cells; Authors - Ting Xu