Adenosine A1 Receptor Polyklonaler Antikörper

Adenosine A1 Receptor Polyklonal Antikörper für FC, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, FC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 20332-1-AP

Synonyme

adenosine A1 receptor, Adenosine receptor A1, ADORA1, RDC7

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Galerie der Validierungsdaten

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  WB analysis of mouse eye using 20332-1-AP

  mouse eye tissue were subjected to SDS PAGE followed by western blot with 20332-1-AP (Adenosine A1 Receptor antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 20332-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20332-1-AP (Adenosine A1 Receptor antibody at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 20332-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20332-1-AP (Adenosine A1 Receptor antibody at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 20332-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 20332-1-AP (Adenosine A1 Receptor antibody at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 20332-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 20332-1-AP (Adenosine A1 Receptor antibody at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of SH-SY5Y using 20332-1-AP

  1X10^6 SH-SY5Y cells were stained with 0.2ug Adenosine A1 Receptor antibody (20332-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Augengewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe, Maus-Cerebellum-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

20332-1-AP bindet in WB, IHC, FC, ELISA Adenosine A1 Receptor und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Adenosine A1 Receptor fusion protein Ag14092
• Vollständiger Name: adenosine A1 receptor
• Berechnetes Molekulargewicht: 326 aa, 37 kDa
• Beobachtetes Molekulargewicht: 39 kDa
• GenBank-Zugangsnummer: BC026340
• Gene symbol: ADORA1
• Gene ID (NCBI): 134
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ADORA1, also named as RDC7, is a receptor for adenosine. It is mediated by G proteins which inhibit adenylyl cyclase. Adenosine is an important mediator of ethanol intoxication and exerts some of its effects via ADORA1 in the central nervous system.

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