ADORA2A Polyklonaler Antikörper

ADORA2A Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(8)

Kat-Nr. : 51092-1-AP

Synonyme

RDC8, ADORA2, Adenosine receptor A2a, adenosine A2a receptor

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  WB analysis of mouse brain using 51092-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 51092-1-AP (ADORA2A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 51092-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 51092-1-AP (ADORA2A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 51092-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 51092-1-AP (ADORA2A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 51092-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 51092-1-AP (ADORA2A antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 51092-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 51092-1-AP (ADORA2A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below
• IHC: See 2 publications below

Produktinformation

51092-1-AP bindet in WB, IHC, ELISA ADORA2A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ADORA2A fusion protein Ag0470
• Vollständiger Name: adenosine A2a receptor
• Berechnetes Molekulargewicht: 412 aa, 45 kDa
• Beobachtetes Molekulargewicht: 40-45 kDa
• GenBank-Zugangsnummer: BC013780
• Gene symbol: ADORA2A
• Gene ID (NCBI): 135
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Adenosine (ADO) is a retaliatory metabolite that is expressed in conditions of injury or stress. Its physiological functions are mediated through interaction with four specific transmembrane receptors called ADORA1, ADORA2A, ADORA2B, and ADORA3 (PMID:23994158). ADORA2A (adenosine A2A receptor) has been shown to dampen inflammatory responses. ADORA2A signaling has been discovered on polymorphonuclear neutrophils (PMNs) (PMID:36009485).

Publikationen

Species	Application	Title
rat	WB	Mol Neurobiol; Ketamine counteracts sevoflurane-induced depressive-like behavior and synaptic plasticity impairments through the adenosine A2A receptor/ERK pathway in rats; Authors - Weiwei Yu
rat	WB,IHC	Pediatr Res; Caffeine treatment started before injury reduces hypoxic-ischemic white-matter damage in neonatal rats by regulating phenotypic microglia polarization.; Authors - Liu Yang
human	WB	Ophthalmic Res; 7-Methylxanthine Influences the Behavior of ADORA2A-DRD2 Heterodimers in Human Retinal Pigment Epithelial Cells.; Authors - Yuzhuo Fan
mouse		Adv Sci (Weinh); Targeting the Negative Feedback of Adenosine-A2AR Metabolic Pathway by a Tailored Nanoinhibitor for Photothermal Immunotherapy.; Authors - Yiqiong Liu
mouse	WB	J Pharm Anal; Inosine: a broad-spectrum anti-inflammatory against SARS-CoV-2 infection-induced acute lung injury via suppressing TBK1 phosphorylation; Authors - Ningning Wang
human	WB	Front Pediatr; Analysis and comparisons of gene expression changes in patient- derived neurons from ROHHAD, CCHS, and PWS; Authors - A Kaitlyn Victor