ADORA2A Polyklonaler Antikörper

ADORA2A Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 51092-1-PBS

Synonyme

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  WB analysis of mouse brain using 51092-1-AP (same clone as 51092-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 51092-1-AP (ADORA2A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51092-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 51092-1-AP (same clone as 51092-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 51092-1-AP (ADORA2A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51092-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 51092-1-AP (same clone as 51092-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 51092-1-AP (ADORA2A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51092-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 51092-1-AP (same clone as 51092-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 51092-1-AP (ADORA2A antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 51092-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 51092-1-AP (same clone as 51092-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 51092-1-AP (ADORA2A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 51092-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

51092-1-PBS bindet in WB, IHC, Indirect ELISA ADORA2A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ADORA2A fusion protein Ag0470
• Vollständiger Name: adenosine A2a receptor
• Berechnetes Molekulargewicht: 412 aa, 45 kDa
• Beobachtetes Molekulargewicht: 40-45 kDa
• GenBank-Zugangsnummer: BC013780
• Gene symbol: ADORA2A
• Gene ID (NCBI): 135
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Adenosine (ADO) is a retaliatory metabolite that is expressed in conditions of injury or stress. Its physiological functions are mediated through interaction with four specific transmembrane receptors called ADORA1, ADORA2A, ADORA2B, and ADORA3 (PMID:23994158). ADORA2A (adenosine A2A receptor) has been shown to dampen inflammatory responses. ADORA2A signaling has been discovered on polymorphonuclear neutrophils (PMNs) (PMID:36009485).