ADPGK Polyklonaler Antikörper

ADPGK Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 15639-1-AP

Synonyme

RbBP 35, PSEC0260, EC:2.7.1.147, ADP-GK, ADP-dependent glucokinase

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  WB analysis using 15639-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15639-1-AP (ADPGK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 15639-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15639-1-AP (ADPGK antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 15639-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15639-1-AP (ADPGK antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 15639-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ADPGK antibody (15639-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, MOLT-4-Zellen
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IF: See 1 publications below

Produktinformation

15639-1-AP bindet in WB, IHC, IF/ICC, ELISA ADPGK und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ADPGK fusion protein Ag8105
• Vollständiger Name: ADP-dependent glucokinase
• Berechnetes Molekulargewicht: 497 aa, 54 kDa
• Beobachtetes Molekulargewicht: 51 kDa
• GenBank-Zugangsnummer: BC006112
• Gene symbol: ADPGK
• Gene ID (NCBI): 83440
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ADP-dependent glucokinase (ADPGK) has frst been described 1994 in hyperthermophilic archaea as a novel glucose-phosphorylating enzyme dependent on ADP (adenosine diphosphate) instead of ATP (adenosine triphosphate). Highest ADPGK expression is found in immune cells of both myeloid and lymphoid lineages. Catalyzes the phosphorylation of D-glucose to D-glucose 6-phosphate using ADP as the phosphate donor. GDP and CDP can replace ADP, but with reduced efficiency (By similarity).

Publikationen

Species	Application	Title
human	WB, IF	Front Mol Neurosci; Quantitative Proteomic Analysis of Mouse Sciatic Nerve Reveals Post-injury Upregulation of ADP-Dependent Glucokinase Promoting Macrophage Phagocytosis.; Authors - Kai Zhang
human	WB	Res Sq; Molecular and clinical characterization of a founder mutation causing G6PC3 deficiency; Authors - Xin Zhen
human	WB	J Clin Immunol; Molecular and Clinical Characterization of a Founder Mutation Causing G6PC3 Deficiency; Authors - Xin Zhen
mouse	WB	Biomaterials; P-Pev: micelle-like complexes transformed from tumor extracellular vesicles by PEG-PE for personalized therapeutic tumor vaccine; Authors - Hongjian Tian