ARH3 Polyklonaler Antikörper

ARH3 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 16504-1-AP

Synonyme

ADP-ribosylhydrolase 3, ADP-ribose glycohydrolase ARH3, ADPRHL2, ADPRHL 2, [Protein ADP-ribosylserine] hydrolase

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  WB analysis using 16504-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16504-1-AP (ARH3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 16504-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16504-1-AP (ARH3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 16504-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 16504-1-AP (ARH3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 16504-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 16504-1-AP (ARH3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 16504-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16504-1-AP (ARH3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16504-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16504-1-AP (ARH3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 16504-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16504-1-AP (ARH3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 16504-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 16504-1-AP (ARH3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 16504-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16504-1-AP (ARH3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 16504-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16504-1-AP (ARH3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 16504-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16504-1-AP (ARH3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 16504-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16504-1-AP (ARH3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 16504-1-AP

  Immunofluorescent analysis of HepG2 cells using 16504-1-AP (ARH3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, HeLa-Zellen, HepG2-Zellen, Mausherzgewebe, Mausnierengewebe, Mauslungengewebe, Maus-Thymusgewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: Maushodengewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IP: See 1 publications below

Produktinformation

16504-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA ARH3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ARH3 fusion protein Ag9683
• Vollständiger Name: ADP-ribosylhydrolase like 2
• Berechnetes Molekulargewicht: 363 aa, 39 kDa
• Beobachtetes Molekulargewicht: 39 kDa
• GenBank-Zugangsnummer: BC014169
• Gene symbol: ARH3
• Gene ID (NCBI): 54936
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ARH3 can be detected as two bands between 39 and 40 kDa.(PMID: 16278211/32636369)

Publikationen

Species	Application	Title
human,rat	WB,IP	Nat Commun; Cis- and trans-resveratrol have opposite effects on histone serine-ADP-ribosylation and tyrosine induced neurodegeneration.; Authors - Megha Jhanji