ADRA1B Polyklonaler Antikörper

ADRA1B Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Rezensionen (2)

Kat-Nr. : 22419-1-AP

Synonyme

ADRA1, Alpha 1B adrenergic receptor, Alpha 1B adrenoceptor, Alpha 1B adrenoreceptor, Alpha-1B adrenergic receptor

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• 

  WB analysis using 22419-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22419-1-AP (ADRA1B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  FC experiment of PC-3 using 22419-1-AP

  1x10^6 PC-3 cells were intracellularly stained with 0.8 ug ADRA1B Polyclonal antibody (22419-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.8 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Mausherzgewebe, Maushodengewebe, Rattenhirngewebe
• Erfolgreiche Detektion in FC (Intra): PC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 2 publications below

Produktinformation

22419-1-AP bindet in WB, IHC, ELISA ADRA1B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ADRA1B fusion protein Ag18138
• Vollständiger Name: adrenergic, alpha-1B-, receptor
• Berechnetes Molekulargewicht: 520 aa, 57 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC136569
• Gene symbol: ADRA1B
• Gene ID (NCBI): 147
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-affinitätsgereinigt
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
rat	WB,IHC	Chin Med; Neurobehavioral alternations of the female offspring born to polycystic ovary syndrome model rats administered by Chinese herbal medicine.; Authors - Xian Zhang
rat	WB	Eur J Pharmacol; Alpha1B-adreneroceptor is involved in norepinephrine-induced pulmonary artery smooth muscle cell proliferation via p38 signaling; Authors - Xiaolin Xiao
human	WB	Mol Med Rep; Stigmasterol exerts antiglioma effects by regulating lipid metabolism; Authors - Ting Wei
human	IHC	Onco Targets Ther; Sympathetic innervation contributes to perineural invasion of salivary adenoid cystic carcinoma via the β2-adrenergic receptor.; Authors - Chao Ma

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Andrea (Verified Customer) (07-31-2023)

Good signal in Flow cytometry for endogenous expressing levels and confirmed in overexpressing cell line

• Applications: Flow Cytometry
• Primary Antibody Dilution: 0.4ug/ml

FH

Mohamad (Verified Customer) (07-03-2023)

different bands at different MW

• Applications: Western Blot
• Primary Antibody Dilution: 1/100
• Cell Tissue Type: Lung