ADRA2A Polyklonaler Antikörper

ADRA2A Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(14)

Kat-Nr. : 14266-1-AP

Synonyme

ADRA2, ADRA2R, ADRAR, Alpha 2A adrenergic receptor, Alpha 2A adrenoceptor

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  WB analysis using 14266-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14266-1-AP (ADRA2A antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-12 using 14266-1-AP

  PC-12 cells were subjected to SDS PAGE followed by western blot with 14266-1-AP (ADRA2A Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 14266-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14266-1-AP (ADRA2A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 14266-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14266-1-AP (ADRA2A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, PC-12-Zellen, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 10 publications below
• IHC: See 2 publications below

Produktinformation

14266-1-AP bindet in WB, IHC, ELISA ADRA2A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ADRA2A fusion protein Ag5562
• Vollständiger Name: adrenergic, alpha-2A-, receptor
• Berechnetes Molekulargewicht: 49 kDa
• Beobachtetes Molekulargewicht: 51 kDa
• GenBank-Zugangsnummer: BC050414
• Gene symbol: ADRA2A
• Gene ID (NCBI): 150
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ADRA2A (Adrenoceptor Alpha-2) belongs to the adrenoceptor alpha-2 family of GPCRs coupled to the inhibitory G-alpha subunit (Gi) that suppresses adenylyl cyclase activity, thereby suppressing the cyclization of AMP to cAMP (PMID: 38132444). ADRA2A inhibits the flow of sympathetic nerves in the central nervous system and plays a major role in sedation, anxiolysis, and antinociception (PMID: 33915198).

Publikationen

Species	Application	Title
		Biomed Pharmacother; Dexmedetomidine facilitates autophagic flux to promote liver regeneration by suppressing GSK3β activity in mouse partial hepatectomy; Authors - Xueya Yao
rat	WB	Int J Mol Sci; Chronic Administrations of Guanfacine on Mesocortical Catecholaminergic and Thalamocortical Glutamatergic Transmissions.; Authors - Kouji Fukuyama
human	WB	Am J Cancer Res; SNHG15, a p53-regulated lncRNA, suppresses cisplatin-induced apoptosis and ROS accumulation through the miR-335-3p/ZNF32 axis.; Authors - Yue-Feng Sun
mouse	IHC	Front Immunol; β2-Adrenoceptor Deficiency Results in Increased Calcified Cartilage Thickness and Subchondral Bone Remodeling in Murine Experimental Osteoarthritis.; Authors - Gundula Rösch
mouse	WB	Front Mol Neurosci; PMID: 28955200; Authors - Yong-Jie Wang
mouse	WB	Exp Neurol; Two-hour acute restraint stress facilitates escape behavior and learning outcomes through the activation of the Cdk5/GR P S211 pathway in male mice.; Authors - Bin Wang