ADRM1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

ADRM1 Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

10

Kat-Nr. : 11468-1-AP

Synonyme

adhesion regulating molecule 1, ADRM1, ARM 1, ARM1, GP110, hRpn13, Rpn13, Rpn13 homolog

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 11468-1-AP

  WB result of ADRM1 antibody (11468-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ADRM1 transfected HeLa cells.

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  WB analysis using 11468-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11468-1-AP (ADRM1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 11468-1-AP

  IP Result of anti-ADRM1 (IP:11468-1-AP, 3ug; Detection:11468-1-AP 1:500) with mouse testis tissue lysate 8000ug.

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  IHC staining of human colon cancer using 11468-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11468-1-AP (ADRM1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 11468-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11468-1-AP (ADRM1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 11468-1-AP

  Immunofluorescent analysis of MCF-7 cells, using ADRM1 antibody 11468-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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  FC experiment of MCF-7 using 11468-1-AP

  1X10^6 MCF-7 cells were stained with 0.2ug ADRM1 antibody (11468-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, K-562-Zellen, NIH/3T3-Zellen, PC-3-Zellen, Raji-Zellen
• Erfolgreiche IP: Maushodengewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: MCF-7-Zellen
• Erfolgreiche Detektion in FC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:20-1:200
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 6 publications below
• IHC: See 5 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

11468-1-AP bindet in WB, IP, IHC, IF, FC, CoIP, ELISA ADRM1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ADRM1 fusion protein Ag1997
• Vollständiger Name: adhesion regulating molecule 1
• Berechnetes Molekulargewicht: 407 aa, 42 kDa
• Beobachtetes Molekulargewicht: 46 kDa
• GenBank-Zugangsnummer: BC017245
• Gene symbol: ADRM1
• Gene ID (NCBI): 11047
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The 26S proteasome is a key component of the ubiquitin-proteasome system, a process responsible for the majority of cellular protein degradation. hRpn13 (also termed ADRM1 or GP110) is a novel 46-kDa subunit of its 19S regulatory complex. hRpn13 binds directly to the proteasome-associated deubiquitinating enzyme, UCH37, and enhances its isopeptidase activity. Overexpression of hRpn13 promotes the activity of the ubiquitin-proteasome system and modulates the influence of osteoblasts on osteoclasts by controlling the stability of regulatory proteins in osteoblasts.

Publikationen

Species	Application	Title
human	WB	Mol Cell; An Interaction Landscape of Ubiquitin Signaling.; Authors - Xiaofei Zhang
mouse	IHC	Proc Natl Acad Sci U S A; Dendritic spinopathy in transgenic mice expressing ALS/dementia-linked mutant UBQLN2.; Authors - George H Gorrie
	WB	Cell Rep; SUMOylation of Psmd1 Controls Adrm1 Interaction with the Proteasome.; Authors - Hyunju Ryu
human	WB	Oncogene; The degradation of p53 and its major E3 ligase Mdm2 is differentially dependent on the proteasomal ubiquitin receptor S5a.; Authors - Sparks A A
human	IHC	Genes Chromosomes Cancer; Comprehensive analysis of 20q13 genes in ovarian cancer identifies ADRM1 as amplification target.; Authors - Fejzo Marlena S MS
mouse	IF	Biochem Biophys Res Commun; Disturbance of proteasomal and autophagic protein degradation pathways by amyotrophic lateral sclerosis-linked mutations in ubiquilin 2.; Authors - Mayuko Osaka

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