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Perilipin-2 Polyklonaler Antikörper

Perilipin-2 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (6)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(189)

Kat-Nr. : 15294-1-AP

Synonyme

ADFP, Adipophilin, Adipose differentiation-related protein, ADRP, ADRP/Perilipin 2



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHepG2-Zellen, 3T3-L1-Zellen, K-562-Zellen, Mauslebergewebe, NIH/3T3-Zellen, Rattenlebergewebe
Erfolgreiche Detektion in IHChumanes Leberkarzinomgewebe, humanes Kolonkarzinomgewebe, humanes Lebergewebe, humanes Prostatakarzinomgewebe, humanes Nierenzellkarzinomgewebe, Mauslebergewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCmit Ölsäure behandelte HeLa-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:2000-1:16000
Immunhistochemie (IHC)IHC : 1:200-1:8000
Immunfluoreszenz (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

15294-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA Perilipin-2 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Affe, Hausschwein, Hund, Maus, Ratte, Rind, Zebrafisch, Ziege
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Perilipin-2 fusion protein Ag7539
Vollständiger Name adipose differentiation-related protein
Berechnetes Molekulargewicht 48 kDa
Beobachtetes Molekulargewicht 45-48 kDa
GenBank-ZugangsnummerBC005127
Gene symbol Perilipin-2
Gene ID (NCBI) 123
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ADRP (adipocyte differentiation related protein) also known as ADFP, adipophilin, or perilipin-2, is a member of PAT family which is responsible for the transportation of lipids and the formation of lipid droplets. ADRP is localized on the surface of lipid droplets in a variety of tissues and cell lines. ADRP is not detected in undifferentiated cells but increases rapidly to high levels when adipocyte precursors differentiate into adipocytes. Anti-ADRP antibody is a reliable and sensitive marker for lipid droplet. Enhanced expression of ADRP is linked to diseases with abnormal lipid storage, including hepatic steatosis, atherosclerosis and diabetes. Immunohistochemistry of ADRP may facilitate histomorphological diagnosis of these diseases. This antibody is not suitable for immunofluorescence staining of frozen sections.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for Perilipin-2 antibody 15294-1-APProtokoll herunterladen
IHC protocol for Perilipin-2 antibody 15294-1-APProtokoll herunterladenl
IF protocol for Perilipin-2 antibody 15294-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanIF

Nature

iPS-cell-derived microglia promote brain organoid maturation via cholesterol transfer

Authors - Dong Shin Park
humanIF

Nature

The role of NSP6 in the biogenesis of the SARS-CoV-2 replication organelle.

Authors - Simona Ricciardi
mouseIHC

Signal Transduct Target Ther

The AKAP12-PKA axis regulates lipid homeostasis during alcohol-associated liver disease

Authors - Chandana Thimme Gowda
mouse,humanIHC,IF

Cell Metab

Elevation of JAML Promotes Diabetic Kidney Disease by Modulating Podocyte Lipid Metabolism.

Authors - Yi Fu
mouseIF

Nat Commun

Spliceosome component Usp39 contributes to hepatic lipid homeostasis through the regulation of autophagy

Authors - Donghai Cui
mouseWB

Brain Behav Immun

HIV-TAT dysregulates microglial lipid metabolism through SREBP2/miR-124 axis: Implication of lipid droplet accumulation microglia in NeuroHIV

Authors - Yan Cheng

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Samuel (Verified Customer) (07-31-2025)

I found this primary antibody to work quite well, at least from a Western blot perspective, (I have not tried any other applications using this particular antibody). The experimental results came out quite clean and there has been little to no non-specific banding patterns, which is very nice. Lastly, the standard 1:1000 primary antibody dilution I have used works just fine for my needs, which allows the vial to last a decent amount of time. Overall, I would recommend!

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
FH

Christin (Verified Customer) (11-25-2024)

Excellent antibody for ICC and WB of human iPSC-derived microglia and macrophages

  • Applications: Western Blot, Immunofluorescence
  • Primary Antibody Dilution: 1:250
  • Cell Tissue Type: Human iPSC-derived microglia and macrophages
FH

Liliana (Verified Customer) (02-13-2024)

WORKS VERY WELL ON PH9, 1:2000 AND ON PH6, 1:1000

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: SKIN WITH SEBACCEUS ADENOMA
Perilipin-2 Antibody Immunohistochemistry validation (1:2000 dilution) in SKIN WITH SEBACCEUS ADENOMA (Cat no:15294-1-AP)
FH

Christine (Verified Customer) (09-19-2023)

Tried on HEK293T cells treated with 300 uM oleic acid overnight to induce the formation of lipid droplets. This antibody decorates some droplets but was a bit weak. TIP47 antibody (10694-1-AP) performed better (clearer detection of the droplets) at the same concentration (1:100).

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: HEK293T
FH

Kamal (Verified Customer) (07-05-2023)

Worked well when diluted with 1X PBS.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: Mouse Liver tissue
FH

Christin (Verified Customer) (03-09-2023)

Great antibody for WB with low background signal.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: human iPSC-derived microglia and macrophages
FH

Nick (Verified Customer) (04-20-2022)

Helped localize expression of Plin2

  • Applications: Western Blot, Immunoprecipitation, Cell culture
  • Cell Tissue Type: 3T3-L1
FH

Boyan (Verified Customer) (05-09-2019)

Very good for WB

  • Applications: Western Blot,
  • Cell Tissue Type: MEF