Perilipin-2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Perilipin-2 Polyklonal Antikörper für IF/ICC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF/ICC

Konjugation

CoraLite®594 Fluorescent Dye

Publikationen(2)

Kat-Nr. : CL594-15294

Synonyme

ADFP, Perilipin 2, ADRP/Perilipin 2, ADRP, Adipose differentiation-related protein

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  IF Staining of HeLa using CL594-15294

  Immunofluorescent analysis of (-20°C Ethanol) fixed oleic acid treated HeLa cells using CoraLite®594 ADRP/Perilipin 2 antibody (CL594-15294) at dilution of 1:400, CL488-Phalloidine (green).

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  IF Staining of HeLa using CL594-15294

  Immunofluorescent analysis of (-20°C Ethanol) fixed oleic acid treated HeLa cells using CoraLite®594 Perilipin-2 antibody (CL594-15294) at dilution of 1:200.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: mit Ölsäure behandelte HeLa-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 2 publications below

Produktinformation

CL594-15294 bindet in IF/ICC Perilipin-2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Perilipin-2 fusion protein Ag7539
• Vollständiger Name: adipose differentiation-related protein
• Berechnetes Molekulargewicht: 48 kDa
• Beobachtetes Molekulargewicht: 45-48 kDa
• GenBank-Zugangsnummer: BC005127
• Gene symbol: Perilipin-2
• Gene ID (NCBI): 123
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ADRP (adipocyte differentiation related protein) also known as ADFP, adipophilin, or perilipin-2, is a member of PAT family which is responsible for the transportation of lipids and the formation of lipid droplets. ADRP is localized on the surface of lipid droplets in a variety of tissues and cell lines. ADRP is not detected in undifferentiated cells but increases rapidly to high levels when adipocyte precursors differentiate into adipocytes. Anti-ADRP antibody is a reliable and sensitive marker for lipid droplet. Enhanced expression of ADRP is linked to diseases with abnormal lipid storage, including hepatic steatosis, atherosclerosis and diabetes. Immunohistochemistry of ADRP may facilitate histomorphological diagnosis of these diseases.

Publikationen

Species	Application	Title
	IF	bioRxiv; Cold induces brain region-selective neuronal activity-dependent lipid metabolism; Authors - Hyeonyoung Min
mouse	IF	Elife; Cold induces brain region-selective cell activity-dependent lipid metabolism; Authors - Hyeonyoung Min