ADSL Rekombinanter Antikörper

ADSL Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

250216C6

Kat-Nr. : 86131-1-RR

Synonyme

AMPS, ASASE, ASL, EC:4.3.2.2

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  WB analysis using 86131-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86131-1-RR (ADSL antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 86131-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86131-1-RR against Human ADSL were performed. The affinity constant is 4.05 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HL-60-Zellen, C6-Zellen, NIH/3T3-Zellen, Ramos-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86131-1-RR bindet in WB, ELISA ADSL und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ADSL fusion protein Ag7332
• Vollständiger Name: adenylosuccinate lyase
• Berechnetes Molekulargewicht: 55 kDa
• Beobachtetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: BC000253
• Gene symbol: ADSL
• Gene ID (NCBI): 158
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Adenylosuccinate lyase (ADSL) is an essential enzyme for de novo purine biosynthesis and the purine nucleotide cycle, where it plays a key role in the regulation of adenosine monophosphate levels in the cells. Given its crucial role in both cellular replication and metabolism, it is not surprising that ADSL has been found dysregulated in several malignancies, including breast and endometrial cancers as well as glioma. (PMID: 33754045)