AEG-1/MTDH Monoklonaler Antikörper

AEG-1/MTDH Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1D8C2

Kat-Nr. : 68592-1-PBS

Synonyme

AEG-1, MTDH, 1D8C2, 3D3/LYRIC, AEG 1

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  WB analysis using 68592-1-Ig (same clone as 68592-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68592-1-Ig (AEG-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68592-1-PBS in a different storage buffer formulation.

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  IHC staining of human rectal cancer using 68592-1-Ig (same clone as 68592-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 68592-1-Ig (AEG-1/MTDH antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68592-1-PBS in a different storage buffer formulation.

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  IHC staining of human rectal cancer using 68592-1-Ig (same clone as 68592-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 68592-1-Ig (AEG-1/MTDH antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68592-1-PBS in a different storage buffer formulation.

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  IHC staining of human rectal cancer using 68592-1-Ig (same clone as 68592-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 68592-1-Ig (AEG-1/MTDH antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68592-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 68592-1-Ig (same clone as 68592-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 68592-1-Ig (AEG-1/MTDH antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68592-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 68592-1-Ig (same clone as 68592-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using AEG-1/MTDH antibody (68592-1-Ig, Clone: 1D8C2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68592-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68592-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA AEG-1/MTDH und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: AEG-1/MTDH fusion protein Ag5033
• Vollständiger Name: metadherin
• Berechnetes Molekulargewicht: 582 aa, 64 kDa
• Beobachtetes Molekulargewicht: 70-80 kDa
• GenBank-Zugangsnummer: BC045642
• Gene symbol: AEG-1/MTDH
• Gene ID (NCBI): 92140
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MTDH, also known as AEG-1, is a single-pass transmembrane protein with its gene located at chromosome 8q22 , the abnormalities of which have been identified in several tumor types. MTDH is overexpressed in a number of malignancies, such as breast cancer, malignant glioma, and neuroblastoma , and its overexpression is associated with poor clinical outcome. MTDH plays an important role in the regulation of carcinogenesis and tumor progression.