AEG-1/MTDH Rekombinanter Antikörper

AEG-1/MTDH Rekombinant Antikörper für WB, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

242110B5

Kat-Nr. : 84686-4-RR

Synonyme

AEG-1, MTDH, 242110B5, 3D3/LYRIC, AEG 1

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  WB analysis using 84686-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84686-4-RR (AEG-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 84686-4-RR

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using AEG-1/MTDH antibody (84686-4-RR, Clone: 242110B5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of A549 using 84686-4-RR

  1x10^6 A549 cells were intracellularly stained with 0.25 ug AEG-1/MTDH Recombinant antibody (84686-4-RR, Clone:242110B5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 84686-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84686-4-RR against Human AEG-1/MTDH were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HeLa-Zellen, HepG2-Zellen, HL-60-Zellen, MCF-7-Zellen, U-937-Zellen
• Erfolgreiche Detektion in IF/ICC: A431-Zellen
• Erfolgreiche Detektion in FC (Intra): A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:250-1:1000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84686-4-RR bindet in WB, IF/ICC, FC (Intra), ELISA AEG-1/MTDH und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: AEG-1/MTDH fusion protein Ag4840
• Vollständiger Name: metadherin
• Berechnetes Molekulargewicht: 582 aa, 64 kDa
• Beobachtetes Molekulargewicht: 75 kDa
• GenBank-Zugangsnummer: BC045642
• Gene symbol: AEG-1/MTDH
• Gene ID (NCBI): 92140
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MTDH, also known as AEG-1, is a single-pass transmembrane protein with its gene located at chromosome 8q22 , the abnormalities of which have been identified in several tumor types. MTDH is overexpressed in a number of malignancies, such as breast cancer, malignant glioma, and neuroblastoma , and its overexpression is associated with poor clinical outcome. MTDH plays an important role in the regulation of carcinogenesis and tumor progression.