AES Rekombinanter Antikörper

AES Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

240987D9

Kat-Nr. : 83878-5-RR

Synonyme

TLE5, 240987D9, AES 1, AES 2, Amino-terminal enhancer of split

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  WB analysis using 83878-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83878-5-RR (AES antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83878-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83878-5-RR against Human AES were performed. The affinity constant is 1.76 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, DU 145-Zellen, K-562-Zellen, MDA-MB-231-Zellen, Maus-Skelettmuskelgewebe, NIH/3T3-Zellen, Ratten-Skelettmuskelgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83878-5-RR bindet in WB, ELISA AES und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: AES fusion protein Ag21021
• Vollständiger Name: amino-terminal enhancer of split
• Berechnetes Molekulargewicht: 264 aa, 29 kDa
• Beobachtetes Molekulargewicht: 22-29 kDa
• GenBank-Zugangsnummer: BC113735
• Gene symbol: AES
• Gene ID (NCBI): 166
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Amino-terminal enhancer of split (AES), belongs to the groucho/TLE family of proteins, can function as a homooligomer or as a heteroologimer with other family members in order to forcefully repress the expression of other family member genes. AES can repress NFkB-regulated gene expression and is believed to have a significant role in initiating and maintaining cell differentiation events. AES has been found to suppress local invasion and intravasation through inhibition of the Notch pathway, leading to preventing the metastatic spread of endogenous tumors(PMID: 33754069).