AFAP1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

AFAP1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

Rezensionen (1)

Kat-Nr. : 21093-1-AP

Synonyme

110 kDa actin filament-associated protein, Actin filament-associated protein 1, AFAP, AFAP 110, AFAP-110

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  WB analysis using 21093-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21093-1-AP (AFAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 21093-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21093-1-AP (AFAP1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 21093-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 21093-1-AP (AFAP1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 21093-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 21093-1-AP (AFAP1 Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 21093-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 21093-1-AP (AFAP1 Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A549 using 21093-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using AFAP1 antibody (21093-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of HeLa using 21093-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using AFAP1 antibody (21093-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: DU 145-Zellen, HeLa-Zellen, MCF-7-Zellen, Maushirngewebe, PC-3-Zellen, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen, A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IF: See 1 publications below

Produktinformation

21093-1-AP bindet in WB, IHC, IF/ICC, ELISA AFAP1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AFAP1 fusion protein Ag15346
• Vollständiger Name: actin filament associated protein 1
• Berechnetes Molekulargewicht: 730 aa, 81 kDa
• Beobachtetes Molekulargewicht: 110 kDa
• GenBank-Zugangsnummer: BC032777
• Gene symbol: AFAP1
• Gene ID (NCBI): 60312
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Actin filament-associated protein 1 (AFAP1) encodes a motor fiber-associated protein, which organizes a network for linking other proteins, including SRC proto-oncogene, non-receptor tyrosine kinase (Src) and protein kinase C . A long non-coding RNA, namely AFAP1-antisense RNA 1 (AS1), is transcribed from the antisense strand of this gene and potentially regulates its expression.(PMID: 31086663, PMID: 35193469)

Publikationen

Species	Application	Title
human	WB	Nucleic Acids Res; CSNK2B modulates IRF1 binding to functional DNA elements and promotes basal and agonist-induced antiviral signaling; Authors - Moe Matsumoto ; KD Validated
	WB,IF	Mol Biol Cell; Spatial proximity of proteins surrounding zyxin under force-bearing conditions.; Authors - Joleen S Cheah ; KD Validated
rat	WB	Int J Mol Med; Curculigoside attenuates myocardial ischemia‑reperfusion injury by inhibiting the opening of the mitochondrial permeability transition pore.; Authors - Yanbing Zhao
human	WB	Biol Res; Long non-coding RNA AFAP1-AS1 facilitates tumor growth and promotes metastasis in colorectal cancer.; Authors - Xu Han
human	WB	J Exp Clin Cancer Res; LncRNA AFAP1-AS1 promotes tumorigenesis and epithelial-mesenchymal transition of osteosarcoma through RhoC/ROCK1/p38MAPK/Twist1 signaling pathway.; Authors - Deyao Shi
human	WB	Oncotarget; LncRNA AFAP1-AS1 promotes growth and metastasis of cholangiocarcinoma cells.; Authors - Xiuhui Shi

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Joleen (Verified Customer) (05-04-2019)

Works excellently for WB. Strong signal by 5s exposure and with typical dilution.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1k
• Cell Tissue Type: MDCK GII

AFAP1 Antibody Western Blot, validation (1:1k dilution) in MDCK GII (Cat no:21093-1-AP)