AFAP1L2 Polyklonaler Antikörper

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• KD/KO Validated

AFAP1L2 Polyklonal Antikörper für IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Rezensionen (1)

Kat-Nr. : 17183-1-AP

Synonyme

AFAP1 like protein 2, AFAP1L2, KIAA1914, XB130

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Galerie der Validierungsdaten

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  WB analysis using 17183-1-AP

  A549 were subjected to SDS PAGE followed by western blot with 17183-1-AP (AFAP1L2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 17183-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 17183-1-AP (AFAP1L2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 17183-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 17183-1-AP (AFAP1L2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 17183-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 17183-1-AP (AFAP1L2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse thymus using 17183-1-AP

  IP Result of anti-AFAP1L2 (IP:17183-1-AP, 4ug; Detection:17183-1-AP 1:500) with mouse thymus tissue lysate 2800ug.

• 

  IHC staining of human thymus using 17183-1-AP

  Immunohistochemical analysis of paraffin-embedded human thymus tissue slide using 17183-1-AP (AFAP1L2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thymus using 17183-1-AP

  Immunohistochemical analysis of paraffin-embedded human thymus tissue slide using 17183-1-AP (AFAP1L2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, Maus-Thymusgewebe
• Erfolgreiche IP: Maus-Thymusgewebe
• Erfolgreiche Detektion in IHC: humanes Thymusgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Produktinformation

17183-1-AP bindet in WB, IP, IHC, IF, ELISA AFAP1L2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AFAP1L2 fusion protein Ag10966
• Vollständiger Name: actin filament associated protein 1-like 2
• Berechnetes Molekulargewicht: 818 aa, 91 kDa
• Beobachtetes Molekulargewicht: 130 kDa
• GenBank-Zugangsnummer: BC033212
• Gene symbol: AFAP1L2
• Gene ID (NCBI): 84632
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AFAP1L2, also named as Actin filament-associated protein 1-like 2 or XB130, is a 818 amino acid protein, which contains two PH domains. AFAP1L2 is detected in spleen and thyroid, and at lower levels in kidney, brain, lung and pancreas. AFAP1L2 localizes in the cytoplasm and may play a role in a signaling cascade by enhancing the kinase activity of SRC. AFAP1L2 promotes thyroid tumor growth, and its expression in these cancer cells may affect cell proliferation and survival by controlling the expression of multiple genes, especially transcription regulators.

Publikationen

Species	Application	Title
human	IHC	Transl Oncol; Identification of genes associated with local aggressiveness and metastatic behavior in soft tissue tumors.; Authors - Cunha Isabela Werneck IW
mouse	IHC	Carcinogenesis; XB130 deficiency enhances carcinogen-induced skin tumorigenesis.; Authors - Hae-Ra Cho ; KO Validated
mouse	WB	Mol Cell; PLEKHS1 drives PI3Ks and remodels pathway homeostasis in PTEN-null prostate; Authors - Tamara A M Chessa
human	WB	Oncol Lett; The 3'‑untranslated region of XB130 regulates its mRNA stability and translational efficiency in non‑small cell lung cancer cells; Authors - Qinrong Wang
mouse	IF,WB,IHC	Cell Rep; Integrative multi-omic profiling of adult mouse brain endothelial cells and potential implications in Alzheimer's disease; Authors - Min Yu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tamara (Verified Customer) (12-02-2020)

antibody works very well in detecting endogenous Afap1l2 protein in mouse prostate lysates

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: mouse prostate