AFF2 Polyklonaler Antikörper

AFF2 Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 25414-1-AP

Synonyme

AF4/FMR2 family member 2, FMR2, FMR2P, FRAXE, MRX2

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  WB analysis using 25414-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25414-1-AP (AFF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of SH-SY5Y using 25414-1-AP

  IP result of anti-AFF2 (IP:25414-1-AP, 4ug; Detection:25414-1-AP 1:600) with SH-SY5Y cells lysate 1720 ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SH-SY5Y-Zellen
• Erfolgreiche IP: SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

25414-1-AP bindet in WB, IP, ELISA AFF2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AFF2 fusion protein Ag22225
• Vollständiger Name: AF4/FMR2 family, member 2
• Berechnetes Molekulargewicht: 1311 aa, 145 kDa
• Beobachtetes Molekulargewicht: 145 kDa
• GenBank-Zugangsnummer: BC132683
• Gene symbol: AFF2
• Gene ID (NCBI): 2334
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AFF2 (OMIM* 300806) (also known as FMR2 gene), which encodes AF4/FMR2 family member 2, is a transcriptional factor and RNA-binding protein that plays an important role in transcriptional regulation, RNA splicing, mRNA processing, and nuclear speckle organization . AFF2 is highly conserved and abundantly expressed in human brain, being essential for brain development. Homozygous AFF2 knock-out mice showed abnormal central nervous system synaptic transmission, abnormal excitatory postsynaptic potential, and premature death (PMID: 35431806).